复方木枣口服液对小鼠免疫功能影响的实验研究  

Effect of compound jujube oral liquid on immune function of mouse

作　　者：李兴旺 尹进[2] 邹瑾 

机构地区：[1]汨罗市中医院,湖南汨罗414400 [2]湖南省疾病预防控制中心,湖南长沙410005 [3]湖南科技职业技术学院,湖南长沙410004

出　　处：《中国热带医学》2009年第8期1440-1441,共2页China Tropical Medicine

基　　金：湖南省教育厅资助项目(08D007)

摘　　要：目的探讨复方木枣口服液对小鼠免疫功能的影响。方法采用1.44mL/kg.bw、4.33gmL/kg.bw、12.98mL/kg.bw剂量的复方木枣口服液给小鼠经口灌胃30d,分别采用Jerne改良玻片法进行抗体生成细胞检测、MTT法进行刀豆蛋白A(ConA)诱导的小鼠淋巴细胞转化实验、足趾增厚法进行迟发型变态反应实验(DTH)、测定免疫器官脏器/体重比值、半数溶血值(HC50)和NK细胞活性(同位素3H-TdR测定法)等。结果与对照组比较,中、高剂量组能显著提高小鼠的抗体生成细胞数和高剂量组能显著提高小鼠淋巴细胞转化能力和迟发型变态反应能力,差异均具有统计学意义(P<0.05)。各剂量对小鼠体重增长、胸腺/体重比值、脾脏/体重比值、碳廓清能力、NK细胞活性、半数溶血值及巨噬细胞吞噬鸡红细胞的能力均无显著影响(P>0.05)。结论复方木枣口服液能显著增强小鼠免疫力作用。Objective To discuss the effect of compound jujube oral liquid on immune function in mouse. Methods Compound jujube decoction at doses of 1.44mL/kg.bw,4.33gmL/kg.hw, 12.98mL/kg,bw were given to mice for a course of 30 days,then the quantity of antibedy-predueing cells,lymphocyte transformation were determined and restults of delayed type hypersensitivity,etc,were observed. Results Compared with the control group,The number of antibedy-preducing cells and the ability of lymphocyte transformation and hangfire allergy in mice given compound jujube oral liquid were enhanced （P〈0.05）. There no significant differences were observed in other values between the two groups. Conclusion Compound jujube oral liquid can enhance the immune function of mice.

关 键 词：复方木枣口服液 免疫 小鼠 

分 类 号：R285.5[医药卫生—中药学]