HLA和BA对不明原因反复流产免疫治疗妊娠结局预测  

Comparision of value of human leukocyte antigen gene and blocking antibody in predicting outcome of unexplained recurrent spontaneous abortion treated with paternal lymphocyte alloimmunization therapy

作　　者：郑妍[1] 易冰[1] 季明芳[1] 何庭宇[1] 何茜冬[1] 

机构地区：[1]中山市人民医院,广东中山528400

出　　处：《中国热带医学》2009年第8期1451-1452,共2页China Tropical Medicine

摘　　要：目的探讨人类白细胞抗原(HLA)基因及封闭抗体(Blocking Antibodies,BA)对不明原因反复流产(URSA)免疫治疗疗效的预测价值。方法免疫治疗前用SSP-PCR方法测定32例URSA患者的HLA-A、B、DR、及G的表达率,并随访妊娠结局。用酶联免疫法(ELISA)对52例URSA患者测定免疫治疗后封闭抗体(BA)转阳情况并随访阳性者与阴性者的妊娠率。同时测定同期正常妊娠56例与免疫治疗后成功妊娠者40例中BA阳性率。结果HLA-G*010401及HLA*1502的表达频率在再次流产者中明显高于成功妊娠者,免疫治疗后封闭抗体阳性者及阴性者的妊娠成功率差异无统计学意义;正常妊娠者与免疫治疗后成功妊娠者封闭抗体阳性率差异也无统计学意义。结论HLA-G*010401,HlA-DRB1*1502与免疫治疗后妊娠结局关系密切,有可能成为有价值的预测指标,而封闭抗体无预测价值。Object To compare the predictive value of the human leukocyte antigen gene （HLA）and blocking antidody（ BA ）for the outcome of URSA treated with paternal lymphocyte alloinnnunization therapy（PLATO. Method 52 URSA patients were enrolled in our study.the frequencies of HLA-G＊010401,DRBl＊1502,A＊2402 and B＊5201 were measured by SSP-PCRin 32 patients ,BA were tested by ELISA before and after PLAT.We followed up pregnancy outcome to compare the frequencies of HLA and BA between the successful cases and aborted cases.At the same time,we measured BA in 56 normal pregnancy women to compare the frequencies of BA between normal pregnancy and pregnancy treated with PLAT.

关 键 词：人类白细胞抗原基因 封闭抗体 流产 淋巴细胞免疫治疗 

分 类 号：R714.2[医药卫生—妇产科学]