IL-12真核表达质粒对小鼠气道变应性炎症的免疫调节作用  

Immunological effects of eukaryotic expression plasmid psIL-12 on allergic airway inflammation in a murine model

作　　者：黄江菊[1] 朱道银[2] 洪苏玲[1] 陈全[2] 

机构地区：[1]重庆医科大学附属第一医院耳鼻喉科,重庆400016 [2]重庆医科大学基础医学院免疫学教研室,重庆400016

出　　处：《第四军医大学学报》2009年第15期1367-1370,共4页Journal of the Fourth Military Medical University

基　　金：国家"十五"科技攻关项目(2004BA720A19-02)

摘　　要：目的:研究IL-12真核表达质粒(psIL-12)在气道内表达对成年小鼠气道变态反应炎症的影响.方法:以卵白蛋白(OVA)复制成年小鼠气道变态反应炎症模型,气道内应用单剂量psIL-12,pcDNA3.1(+),PBS/脂质体复合物,同时设正常对照组.分别检测肺单链IL-12表达,计数支气管肺泡灌注液(BALF)中嗜酸粒细胞的百分比和IL-4,IFN-γ含量,观察肺组织形态学变化.结果:气道转染psIL-12可在小鼠肺组织中表达IL-12mRNA;psIL-12干预组BALF中嗜酸粒细胞百分比为(2.6±2.3)%,较PBS对照组(11.6±2.3)%明显降低,两组组间差异具有显著性(P<0.05).psIL-12干预组BALF中IL-4含量为(103±53)ng/L,IFN-γ含量为(171±20)ng/L,而PBS对照组IL-4含量为(319±12)ng/L,IFN-γ含量为(77±36)ng/L,两组组间差异具有统计学意义(P<0.05).psIL-12可明显阻止模型鼠肺组织病理改变,降低血清中OVA特异性IgE抗体含量.结论:psIL-12转染气道表达IL-12,增加Th1型细胞因子IFN-γ的产生,抑制抗原激发的Th2型细胞因子IL-4产生,降低血清中OVA特异性IgE抗体含量,能明显抑制气道变态反应性炎症,有望成为治疗变应性鼻炎和支气管哮喘的新方法.AIM： To observe the immunological effects of eukaryotic expression plasmid psIL-12 on airway allergic inflammation in an ovalbumin-induced （OVA） murine model. METHODS： BALB/c mice were randomized into 4 groups： psIL-12 group, pcDNA3.1 （ ＋ ）group, PBS/Lipofectin complexment group and the healthy control group. The pathological manifestations of the lungs, the expression of IL-12 mRNA in the lung tissues, the eosinophils count, the levels of IL-4, IFN-γ in broncho-alveolar fluid（BALF） and the level of OVA-specific IgE in serum were detected. RESULTS ： It was observed that large numbers of eosinophils infiltrated the lung tissues in the pathological sections of PBS/Lipofectin complexment group, significantly higher than that in psIL-12 group. The IL-12 mRNA was positively expressed in psIL-12 group, but negatively expressed in the other groups. The percentage of eosinophil （2.6 -± 2.3 ） % , the level of IL-4 （103 ± 53 ） ng/L and the level of OVA-specific IgE in BALF of psIL-12 group decreased significantly compared with those in the control group（P 〈0.05）, while the level of IFN-γ （171 ±20） ng/L increased significantly （ P 〈 0.05 ） in psIL-12 group. CONCLUSION： psIL-12 exerts some therapeutic effect through modulating the balance between TH1 and TH2 immune response by increasing the level of IFN-γ and decreasing IL-4 and specific IgE levels. It can be used as a novel treatment for allergic rhinitis and asthma.

关 键 词：白细胞介素12 质粒 变应性鼻炎 哮喘 

分 类 号：R56[医药卫生—呼吸系统]