慢性乙型肝炎患者外周血单核细胞TLR7 mRNA表达与血清IL-12,HBV-DNA载量的相关性  

Correlation between expression of TLR7 mRNA in peripheral blood monouclear cells of chronic hepatitis B patients and IL-12,HBV-DNA load

作　　者：段乃荣[1] 陈红[1] 王岐洁[1] 

机构地区：[1]兰州大学第一医院感染病科,甘肃兰州730000

出　　处：《第四军医大学学报》2009年第15期1422-1424,共3页Journal of the Fourth Military Medical University

摘　　要：目的:研究TLR7在慢性乙型肝炎患者外周血单核细胞的表达及其与IL-12,HBV-DNA载量之间的关系,探讨TLR7在抗病毒免疫应答中的作用机制及意义.方法:用实时荧光定量PCR法检测38例具有不同HBV-DNA载量的慢性乙型肝炎患者和12例正常对照者外周血单核细胞TLR7mR-NA表达,患者按病毒载量分为低、中、高3组.实时荧光定量PCR法测定血清病毒载量,ELISA法测定血清IL-12的水平,多组均数间的比较用方差分析,直线相关性分析用Spearman秩相关.结果:患者组中TLR7mRNA水平比正常对照组显著降低(P<0.01),3组患者间TLR7mRNA表达有显著差异且与血清HBV-DNA载量呈负相关,患者组血清IL-12比正常人显著增高,3组患者外周血清IL-12,低、中病毒载量组的差异无统计学意义(P>0.05),其余各组间差异有统计学意义(P<0.01),且与病毒载量呈负相关(r=-0.075,P<0.01).结论:TLR7在慢性乙型肝炎患者外周血单核细胞的表达下降,TLR7与IL-12可能在慢性乙型肝炎中对病毒清除有重要作用,其水平降低可能与疾病的慢性化机制有关.AIM： To investigate the correlation between the expression of TLR7 mRNA in peripheral blood monouclear cells （PBMCs） of chronic hepatitis B patients and IL-12 and HBVDNA load and to explore the role of TLR7 in anti-HBV-DNA. METHODS： TLR7 mRNA expression was examined by real-time quantitative PCR in 38 patients with different HBV-DNA loads and 12 healthy controls, and the patients were divided into 3 groups based on low, medium or high viral load. HBV-DNA was examined by real-time quantitative PCR and the level of serum interleukin-12（ IL-12 ） was examined by ELISA. The difference of mean between groups was studied by variance analysis and the correlation between HBV-DNA load and TLR7 expression was analyzed by Spearman rank correlation. RESULTS： The results demonstrated that the expression of TLR7 was significantly lower in HBV infection groups than that in control groups, with statistically significant difference （ P 〈 0. 01 ）. There was statistical difference in TLR7 mRNA expression among the 3 groups of chronic hepatitis B patients, and the expression of TLR7 was negatively correlated with HBVDNA load. The level of serum IL- 12 was significantly higher in HBV infection groups than that in controls. There was no statistical difference between the groups of low and medium virus load （ P 〉 0.05 ）, while the difference in the difference was statistically significant between other groups （P 〈 0.01 ）. IL-12 was negatively correlated with HBV-DNA load （r = - 0. 075, P 〈 0. 01 ）. CONCLUSION： TLR7 mRNA expression is down-regulated in PBMCs of HBV-infected patients. Both TLR7 and IL-12 play some important roles in clearing out virus and the down-regulation of them may relate to the chronic mechanism of hepatitis B.

关 键 词：TOLL样受体7 白介素-12 病毒载量 免疫 天然 慢性乙型肝炎 

分 类 号：R512.6[医药卫生—内科学]