毛竹种子发芽特性和愈伤组织诱导能力初探  

Studies on Germination Property and Callus Induction of Phyllostachys heterocycla var. pubescens Seed

作　　者：李楠[1,2] 金群英[2] 彭华正[2] 华锡奇[2] 汪奎宏[2] 

机构地区：[1]浙江林学院林业与生物技术学院,浙江临安311300 [2]浙江省林业科学研究院,浙江杭州310023

出　　处：《浙江林业科技》2009年第3期73-76,共4页Journal of Zhejiang Forestry Science and Technology

基　　金：国家自然科学基金项目"竹子鞭芽发育基因调控的新研究模型建立及其实例分析(30571519)";国家青年科学基金项目"PpHB1;生长激素和microRNA在竹笋期维管组织发育过程中的分布规律研究(30700635)"资助

摘　　要：以毛竹成熟种子为实验材料,研究了毛竹种子的发芽特性、不同种子处理和蔗糖含量对其发芽的影响,以及影响毛竹种子愈伤组织诱导能力的因素,试验结果表明,毛竹种子胚乳中伴生的真菌是造成毛竹种子组织培养操作污染的主要原因,切除大部分胚乳,能有效减少污染;毛竹种子胚乳的切除并不影响其在培养基中的萌发,适当增加培养基中的蔗糖浓度也不能明显改变其萌发率;种子愈伤组织的诱导率与诱导过程中的种子发芽率无明显相关。Experiment was made on mature Phyllostachys heterocycla var. pubescens seed to study germination property, effect of different endosperm content and sucrose content in culture medium on seed germination, and factors to influence callus induction ability of seed. The results indicated that fungi associated with seed endosperm caused pollution during operation of tissue culture. Therefore, excision the most part of endosperm would make effective disinfection, but had no effect on germination during tissue culture, just like increase sucrose content in the medium. There had no relation between callus induction rate with germination rate of seed in the process of callus induction.

关 键 词：毛竹 种子 发芽特性 愈伤组织 组织培养 

分 类 号：S718[农业科学—林学]