经典药对远志-石菖蒲配伍前后指标成分的变化分析  

Analysis of Diversify in Matched Pair of Polygala Tenuifolia Willd.and Acorus Tatarinowii Schott by HPLC

作　　者：张文娟[1] 郑晓晖[2] 房敏峰[2] 王庆伟[1] 陈焕[2] 李云峰[2] 张琰[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038 [2]西北大学生命科学学院,陕西西安710069

出　　处：《中国药业》2009年第15期6-7,共2页China Pharmaceuticals

基　　金：陕西省社会发展攻关项目;项目编号:2008K16-03

摘　　要：目的研究经典药对远志-石菖蒲配伍对单味药材指标成分的影响。方法采用高效液相色谱(HPLC)法测定远志、石菖蒲及远志-石菖蒲药对远志皂苷元和α-细辛醚的含量。色谱柱为Shimadzu VP-ODS柱(150mm×4.6mm,5μm);远志皂苷元的流动相为乙腈-0.05%磷酸水溶液(43∶57),检测波长为205nm;α-细辛醚的流动相为乙腈-0.05%磷酸水溶液(52∶48),检测波长为257nm。结果远志、远志-石菖蒲中远志皂苷元含量分别为0.392%和0.297%,石菖蒲、远志-石菖蒲中α-细辛醚含量分别为0.0605%和0.0629%。结论远志-石菖蒲配伍后,药对中远志皂苷元的含量显著降低,而α-细辛醚的含量变化不明显。Objective To research the compatibility regularity of the matched pair of Polygala tenuifolia Willd. and Acorus tatarinowii Schott. Methods The mobile phase of senegenin was acetonitrile- 0. 05% phosphoric acid（43 ： 57）, the detective wavelength was 205 nm; the mobile phase of α-asaricin was acetonitrile-0. 05% phosphoric acid（52：48）, the detective wavelength was 257 nm. The flow rate was about 1 mL/min, and the Shimadzu VP- ODS （150 mm × 4. 6 mm, 5 μm） column was used. Results The contents of senegenin in Polygala tenuifolia Willd. and the matched pair of Polygala tenuifolia Willd. and Acorus tatarinowii Schott were 0. 392% and 0. 297% respectively; the contents of α- asaricin in Acorus tatarinowii Schott and the matched pair of Polygala tenuifolia Willd. and Acorus tatarinowii Schott were 0. 060 5% and 0. 062 9% respectively. Conclusion After the match of Polygala tenuifolia Willd. and Acorus tatarinowii Schott, the content of matched pair was decreased obviously, but there was not obviously change of α- asaricin.

关 键 词：高效液相色谱法 药对 远志-石菖蒲 远志皂苷元 Α-细辛醚 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]