木犀草素对大鼠肺成纤维细胞增殖的作用(英文)  

Effects of Luteolin on the Proliferation of Rat Lung Fibroblasts

作　　者：张国敏[1] 龚国清[1] 李林林[1] 杨丽娜[1] 王寅[1] 

机构地区：[1]中国药科大学药理教研室,南京210009

出　　处：《中国天然药物》2009年第4期297-300,共4页

基　　金：supported by the National Natural Science Foundation of China (No. 30623008)~~

摘　　要：目的:研究木犀草素对乳鼠肺成纤维细胞增殖作用的影响。方法:取3d内出生的乳大鼠肺组织制备肺成纤维细胞,经纯化,接种于96孔培养板上,细胞分为正常对照组、醋酸泼尼松龙组、4×10-4、4×10-5、4×10-6、4×10-7mol·L-14个不同浓度的木犀草素实验组,培养一段时间后,观察肺成纤维细胞的形态变化,测定肺成纤维细胞的生长曲线、增殖情况及细胞内超氧化物歧化酶(SOD)活力。结果:木犀草素浓度在4×10-5、4×10-6mol·L-1时,可明显抑制肺成纤维细胞的生长和增殖,且细胞密度减小,细胞间隔变大,这种作用与药物浓度呈正相关性,且使肺成纤维细胞内蛋白质的含量减少,所有木犀草素给药组均可使肺成纤维细胞内SOD活力随剂量的增加而增加,且与正常对照组比较差异均有统计学意义。结论:木犀草素对大鼠肺成纤维细胞的增殖有抑制作用。AIM： To approach the effects of luteolin on proliferation of rat lung fibroblasts. METHODS： The lung fibroblasts were isolated from the lungs of Sprague-Dawley rats at postnatal day 3, and were then passaged to 96-well culture plates. All cells were divide into 6 groups including normal control group, positive control group, 4×10^-4、4×10^-5、4×10^-6、4×10^-7mol·L^-1. After a period of time, the cytomorphology of LFb, drew growth curve, determined superoxide dismutase （SOD） activity were observed. RESULTS： Luteolin at the concentration of 4×10^-5、4×10^-6mol·L^-1 inhibited evidently the growth and proliferation of lung fibroblasts, and enhanced SOD activity. The density, interval and volume of lung fibroblasts became decrescent. The effects, dose-dependently, had obvious statistical significance compared with normal control group. CONCLUSION： Luteolin can inhibit the proliferation of the lung fibroblasts in rat.

关 键 词：木犀草素 肺成纤维细胞 增殖 抑制作用 

分 类 号：R965[医药卫生—药理学]