HPLC-MS/MS法分离鉴定罗通定在大鼠胆汁中的代谢产物(Ⅱ)(英文)  

Identification of Rotundine Metabolites in Rat Bile Using Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrometry(Ⅱ)

作　　者：牟昀雅[1] 余伯阳[1] 

机构地区：[1]中国药科大学中药复方研究室,南京210009

出　　处：《中国天然药物》2009年第4期312-314,共3页

基　　金：supported by the Key project of Ministry of Education (No.02118)~~

摘　　要：目的:采用HPLC-MS/MS法分析鉴定罗通定在大鼠胆汁中的主要代谢产物。方法:罗通定灌胃给予大鼠(120mg·kg-1),收集胆汁样品。色谱分离采用ZorbaxSBC18(150mm×0.5mmI.D.,5μm);流动相:甲醇-水-三乙胺(30:70:0.07,V/V/V);质谱分析采用(+)ESI模式。结果:在大鼠胆汁中观察到4个罗通定代谢产物,初步推测其中2个为罗通定去甲基化后再与硫酸结合的产物。结论:去甲基化后形成硫酸结合物为罗通定在大鼠胆汁中一主要排泄形式。AIM： To identify the main metabolites of rotundine in rat biliary samples. METHOD： Bile samples of rats after an oral administration of rotundine （120 mg·kg^-1） were analyzed by HPLC-MS/MS. The separation was achieved on a Zorbax SB C18 column （150 mm ×0.5mmI.D.,5μm）; mobile phase consisted of methanol-water-triethylamine （30： 70： 0.07, V/V/V）. Positive ESI mode was operated. RESULT： Four metabolites were observed in rat biliary samples, and the structures of two metabolites were tentatively identified as sulfuric acid conjugates of rotundine after demethylation. CONCLUSION： Conjugation with sulfuric acid after demethylation may be one of the main excretion pathways of rotundine in rat bile.

关 键 词：罗通定 胆汁 代谢产物 HPLC-MS/MS 

分 类 号：R917[医药卫生—药物分析学]