川芎嗪对腹腔间隔室综合征致急性肾损伤干预机制的实验研究  

Study on intervention mechanism of tetramethylpyrazine on acute kidney injury induced by abdominal compartment syndrome

作　　者：查渝[1] 王锦权[1] 刘宝[1] 陶晓根[1] 赵劲松[1] 杜庆[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省立医院ICU,合肥230001

出　　处：《中国临床保健杂志》2009年第3期280-282,共3页Chinese Journal of Clinical Healthcare

基　　金：安徽省自然科学基金资助项目(KJ2007B214)

摘　　要：目的探讨川芎嗪(TMP)对腹腔间隔室综合征(ACS)致急性肾损伤的保护作用机制。方法将60只新西兰白兔随机分成5组,每组12只;对照组(C组)、ACS持续2 h组(A2组)和4 h组(A4组),TMP干预2 h组(T2组)和4 h组(T4组)。腹腔灌注氮气制备ACS兔实验动物模型;TMP干预组在实验前1h给予TMP 60 mg/kg静脉注射。实验结束后采血并处死动物,检测相关观察指标。结果ACS兔实验动物模型随着血内毒素浓度升高,血清肌酐(Cr)、尿素氮(BUN)、半胱氨酸蛋白酶抑制剂C(Cys C)和尿Cys C也升高,呈正相关(均P<0.01),尿量则减少,呈负相关(P<0.01)。与A2组比较,T2组实验动物血清Cr、BUN、Cys C、内毒素较低(均P<0.01),尿量、尿Cys C升高(均P<0.01)。与A4组比较,T4组实验动物血清内毒素升高(P<0.01)。结论TMP早期干预可降低ACS实验动物血清内毒素浓度,改善肾小球滤过率和肾小管重吸收功能,对ACS造成的急性肾损伤具有保护作用。Objective To study the protective effect and mechanism of tetramethylpyrazine （TMP） on acute kidney injury caused by abdominal compartment syndrome （ACS）. Methods 60 New Zealand rabbits were randomly divided into 5 groups, i. e. normal control （ C）, ACS 2 hours （ A2 ）, ACS 4 hours （ A4）, TMP therapy ACS 2 hours （T2） and TMP therapy ACS 4 hours （T4） ,with 12 rabbits in each group. ACS model rabbits were made by injecting nitrogen gas into abdominal cavity. 60mg/kg TMP was administered intravenously into the rabbits of Group T2 and T4 on 1 hour before ACS. Serum creatinine （Cr） ,urea nitrogen （BUN） ,Cystatin C （Cys C） ,urinary Cys C and serum endotoxin were detected respectively, and then the rabbits were killed after the experiment. Results In the ACS model rabbits, serum Cr, BUN, Cys C and urinary Cys C levels were all positively correlated with serum endotoxin level （P 〈 0.01 ）. Between urine volume each hour and serum endotoxin level was negatively correlated （ P 〈 0.01 ）. Compared with A2 group, serum Cr, BUN, Cys C and endotoxin levels were higher （ P 〈 0.01 ）, and urine volume each hour and urinary Cys C were lower （P 〈 0.01 ）in T2 group. Serum endotoxin level was higher in T4 group than in A4 group （P 〈 0.01 ）. Conclusion Early intervention of TMP can protect kidney in the rabbit with ACS early by decreasing serum endotoxin level and improving the function of glomerular filtration and renal tubular reabsorption.

关 键 词：肾功能不全 急性 川芎嗪 筋膜间隔综合征 模型 动物 

分 类 号：R285.5[医药卫生—中药学]