利用改进的整体染色与透明技术观察垂柳的幼胚  

The Use of a Improved Whole Stain-clearing Technique for Observations on Younger Embryos of Salix babylonica L.

作　　者：洪亚平[1] 张亚冰[1] 

机构地区：[1]河南科技大学农学院,河南洛阳471003

出　　处：《安徽农业科学》2009年第22期10400-10402,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30770124);河南科技大学SRTP项目

摘　　要：[目的]利用改进的整体染色与透明技术,对垂柳受精后的胚珠进行了整体染色与透明,以观察胚珠内的幼胚形态。[方法]使用稀释的爱氏苏木精染液对垂柳胚珠进行适时染色,然后用生理盐水(或水)冲洗,使用无水乙醇和油镜油的混合液对其进行脱水,用油镜油进行透明、制片。同时,还对整体染色与透明技术的方法进行了讨论。[结果]在普通(明视野)显微镜下,不仅能够观察到垂柳受精后胚珠的层次结构,还能清楚地观察到胚珠内的幼胚形态。垂柳的鱼雷形幼胚具有2枚子叶,偶尔见到具有3枚子叶的鱼雷形幼胚。[结论]与爱氏苏木精染色-冬青油透明技术相比,使用油镜油作为透明剂,制片方法简单,制片时间短,并且具有比较好的观察效果。The paper give a improved whole stain-clearing technique to observed the younger embryos of S. babylonica. After isolated with gum block and stained by diluted Ehrlich＇ s hematoxylin for a proper time, the fertilized ovules were washed with 0.9% sodium chloride （ or water） , dehydrated with mixed so｜vent of absolute alcohol and a few immersion oil （ artificial cedar oil） , and then cleared and mounted in immersion oil. Using an ordinary bright-field microscopy, the younger embryos inside the fertilized ovules were clearly visible. One torpedo-stage embryo with three cotyledons was found. By contrast with Ehrlieh＇ s hematoxylin-methyl salieylate technique, the method used immersion oil replace the methyl salicylate （poisonous and difficult to buy） , and used a simple procedure to wash and dehydrate the stained ovules. This could finish the mounting at about 20 minutes and had better observing results. The paper also discussed the method of whole stain-clearing technique.

关 键 词：垂柳 胚珠 透明剂 整体透明 植物胚胎学 

分 类 号：S688[农业科学—观赏园艺]