一种改进大肠杆菌DH5α转化效率的方法  

A Method to Improve the Transformation Efficiency of E.coli DH5α

作　　者：杜红旗[1] 李晨阳[1] 田璐[1] 

机构地区：[1]郑州轻工业学院,河南郑州450002

出　　处：《安徽农业科学》2009年第22期10420-10420,10423,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]提高大肠杆菌DH5α转化效率。[方法]在其他条件一致的情况下,将5μl的质粒或连接产物加入含有100μl的感受态0.2 ml PCR管和1.5 ml的离心管中,0.2 ml PCR管设置热休克时间梯度和1.5 ml的离心管热休克时间90 s,对大肠杆菌DH5α转化效率进行了比较。[结果]0.2 ml PCR管转化效率高于1.5 ml的离心管,且0.2 ml PCR管热休克30 s时最高。[结论]采用此方法提高了大肠杆菌DH5α的转化效率。[ Objective] The research aimed to improve the transformation efficiency of E. coli DH5α. [ Method ] Under the same other conditions, 5 μl plasmid or legation product was added into 0.2ml PCR tube and 1.5 ml centrifuge tube with 100 μl competent in them. In different thermal shock time, transformation efficiency of E. coli DH5α was compared. [ Result] Transformation efficiency with 0.2 ml PRC reformer tube is better than that with 1.5 ml centrifuge tube and the 0.2 ml PCR reformer tube with 30 seconds＇ thermal shock time gives the best performance of transformation efficiency. [ Conclusion ] Transformation efficiency of E. coli DH5α is effectively improved by this method.

关 键 词：离心管 感受态 CACL2 热休克 转化 

分 类 号：S188[农业科学—农业基础科学]