青岛、威海水域夏冬季表层沉积物细菌多样性的初步研究  

A Preliminary Study on the Bacterial Diversity in Surface Sediments from the Coastal Water of Qingdao and Weihai in Summer and Winter

作　　者：肖慧[1] 张艳[1] 张喆[2,1] 朱琳[1] 游偲[1] 唐学玺[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《中国海洋大学学报（自然科学版）》2009年第4期641-646,共6页Periodical of Ocean University of China

基　　金：山东省908专项项目(SD-908-01-01-05.06);教育部新世纪优秀人才支持计划项目(NCET-05-0597);国家高技术研究发展规划项目(2007AA09Z416);公益性行业(农业)科研专项经费子课题(nyhyzx07-046)资助

摘　　要：以青岛、威海水域夏冬两季表层沉积物中的基因组DNA为模板,扩增沉积物样品中细菌的16S rDNA片段,并构建其相应文库。进行16S rDNA序列分析,并与数据库中的序列进行比对。分析结果表明:2个站位沉积物样品细菌分别属于11个主要细菌门类,包括:变形菌门(Proteobacteria)、浮霉菌门(Plancomycene)、芽单胞菌门(Gemmatimonadetes)、酸杆菌门(Acidobacteria)、硝化螺菌门(Nitrospirae)、疣微菌门(Verrucomicrobial)、拟杆菌门(Bacteroidetes)、绿屈挠菌门(Chlo-roflexi)、厚壁菌门(Firmicutes)、高G+C含量革兰氏阳性菌放线菌门(Actinobacteria)和低G+C含量革兰氏阳性菌梭菌门(Clostridiales)。其中变形菌门是主要类群,且γ-变形菌纲(γ-Proteobacteria)占优势。与QD2站位相比,QD1站位沉积物细菌多样性随季节变化不大。Based on the purified DNA extracted from surface sediment samples from coastal water of Qingdao and Weihai in summer and winter, 16S rDNA fragments of bacteria were PCR amplified and 16S rDNA clone libraries were constructed. Sequence analysis of 16S rDNA, compared with the published sequences in Genbank database and guided by the phylogenetic tree, the bacteria fell into eleven main lineages： Proteobacteria, Plancomycene, Gemmatimonadetes, Acidobacteria, Nitrospirae, Verrucomicrobial, Bacteroidetes, Chloroflexi, Firmicutes, Actinobacteria and Clostridiales. Proteobacteria was the dominant group and the subgroup of γ-Proteobacteria predominated in them. Compared with Station QD2, the seasonal variation of bacteria diversity in the sediment of OD1 seemed to be very small.

关 键 词：沉积物 16SrDNA 细菌 多样性 青岛 威海 

分 类 号：P714.5[天文地球—海洋科学] Q938.1[生物学—微生物学]