海绵微生物的分离培养及一种活性代谢产物的初步研究  

Isolation,Cultivation of a Sponge-Derived Fungus and Studes on Its Bioactive Metabolites

作　　者：方玉春[1] 李凌绪[2] 朱天骄[1] 蔡生新[1] 顾谦群[1] 朱伟明[1] 王长云[1] 郝双红[2] 

机构地区：[1]教育部海洋药物重点实验室,中国海洋大学海洋药物研究所,山东青岛266003 [2]青岛农业大学山东省农业仿生应用工程技术研究中心,山东青岛266109

出　　处：《中国海洋大学学报（自然科学版）》2009年第4期742-744,共3页Periodical of Ocean University of China

基　　金：国家908专项项目(908-01-ST12908-02-05-04);国家高技术研究发展规划项目(2007AA09Z447);国家自然科学基金项目(40776073)资助

摘　　要：研究海绵来源真菌Aspergillus repens发酵产物中的活性代谢产物。分离培养微生物进行活性筛选,采用活性追踪的方法、利用色谱手段分离获得单体化合物,根据波谱分析及其理化性质确定其结构,利用SRB评价单体化合物的抗肿瘤活性,利用纸片法测定单体化合物的抗植物病菌活性。获得1个萘并吡喃酮化合物,其结构鉴定为3,4-二氢-9,10-二羟基-7-甲氧基-3-甲基-1H-萘并[2,3c]吡喃-1-酮(Semivioxanthin)。该化合物对肿瘤细胞A-549、HL-60和BEL-7402有一定抑制作用,对植物致病菌作用较强。海绵微生物代谢产物是发现活性物质重要资源,值得深入研究。To investigate the bioactive metabolites of sponge-derived fungus Aspergillus repens. Sponge-derived fungi were isolated and cultivated. The bioactive compounds were isolated by bioassay-guided separation procedure and their structures were identified by means of spectral analysis and physicochemical properties. Their bioactivities were preliminarily evaluated in vitro by the SRB and paper method. An anthraquinone was isolated and its structure was identified as 3, 4-Dihydro-9, 10-dihydroxy-7-methoxy-3-methyl-lH-naptho [2, 3c] pyran-l-one. The anthraquinone showed moderate cytotoxicity against A-549, HL-60, BEL-7402 and strong fungicidal activity against plant pathogen. Sponge-derived fungi are important resources for drug discovery.

关 键 词：海绵微生物 抗肿瘤 活性追踪分离 植物致病菌 抗菌活性 

分 类 号：P714.5[天文地球—海洋科学] R282.77[医药卫生—中药学]