参芪地黄汤对糖尿病大鼠肾功能作用的实验研究  

Experimental Study on Protective Function of Shenqi Dihuang Decoction on Diabetic Rats＇ Kidney

作　　者：李春芳[1] 栗春敏[2] 杨红欣[2] 代贵江[1] 

机构地区：[1]牡丹江医学院第二附属医院药剂科,牡丹江157009 [2]牡丹江医学院第二附属医院儿科,牡丹江157009

出　　处：《国际中医中药杂志》2009年第4期293-294,共2页International Journal of Traditional Chinese Medicine

摘　　要：目的探讨参芪地黄汤对2型糖尿病大鼠肾脏的保护作用及其机制。方法以低剂量链脲佐菌素（STZ）注射，制作2型糖尿病动物模型。将SD大鼠分为对照组、模型组和治疗组，治疗组用参芪地黄汤灌胃；对照组及模型组给予同等剂量的饮用水。24周后进行肾脏形态学和生化指标的检测，用放免法检测血清血管紧张素Ⅱ（Ang—Ⅱ）、血清和肾组织内皮素（ET）的水平；用免疫组织化学方法测量肾组织局部转化生长因子β1（TGF—β1）的表达。结果与模型组比较，治疗组大鼠的肾功能及肾脏病变明显改善；血清Ang-Ⅱ、血清和肾组织ET水平降低；肾组织局部TGF—β1表达减少。结论参芪地黄汤对2型糖尿病大鼠的肾脏有保护作用，其机制可能与降低血清Ang—Ⅱ、ET、肾组织ET和TGF-β1水平有关。Objective To study the protective function and the mechanism of Shenqi Dihuang decoction on the kidney of type 2 diabetes mellitus rats. Methods Type 2 diabetes mellitus rat models were set up by injection of small dose of streptozotocin （STZ）. The SD rats were randomized into a control group, a model group and a treatment group. The treatment group was given Shenqi Dihuang decoction. The control group and the model group were given the same amount of water. After 24 weeks, renal morphology examination and biochemical indicator were examined. The levels of Ang-Ⅱ and endothelin （ET） in serum were tested by radioimmnnoassay and the level of transforming growth factorβ1 （TGF-Ⅱ） in renal cortex were determined by immunohistochemistry. Results The kidney function and kidney lesions were significantly improved in the treatment group than in the model group. Ang-Ⅱ And ET levels in serum and renal cortex was reduced, and the expression of TGF-β1 in the treatment group was decreased. Conclusion Shenqi Dihuang Decoction has protective effect on the kidney of diabetic rats, the mechanism may be related to its inhibition on the over expression of Ang-Ⅱ, ET and TGF-β1.

关 键 词：参芪地黄汤 糖尿病肾病 血管紧张素Ⅱ 内皮素 转化生长因子Β1 

分 类 号：R285.5[医药卫生—中药学]