枯草芽胞杆菌XF-1粗蛋白抑菌活性初步研究  

Fungistatic activity of crude proteins of Bacillus subtilis XF-1

作　　者：熊国如[1,2] 张翠英[1] 刘庆丰[1] 李顺德[3] 何月秋[1,4] 

机构地区：[1]云南农业大学,教育部农业生物多样性与病虫害控制重点实验室,昆明650201 [2]中国热带农业科学院生物技术研究所,海口571101 [3]云南省玉溪市农业局种植业处,653100 [4]云南农业大学农学与生物技术学院,昆明650201

出　　处：《植物保护》2009年第4期92-95,共4页Plant Protection

基　　金：云南省重点基金项目;科技部国际科技合作项目(2008GR0999)

摘　　要：枯草芽胞杆菌XF-1是一株对大白菜根肿病有良好防治效果及对多种植物病原真菌有抑菌效果的生防菌株。为了研究其可能的作用机制,本文用硫酸铵沉淀法提取了发酵液中粗蛋白,并在不同处理条件下对其抑菌活性进行了初步研究。该粗蛋白对蛋白酶K和胰蛋白酶及紫外照射均不敏感,对热稳定。经40、60、80℃和100℃处理20 min后,其抑菌作用基本上无变化;经121℃处理20 min后,抑菌活性保持60%。这些结果,为进一步研究XF-1菌株对根肿病的防治机制和进一步应用提供了试验依据。Bacillus subtilis XF-1 is a bioagent with good effects of controlling the clubroot of Chinese cabbage and suppressing many fungal pathogens. To understand its mechanisms of fungistasis, its crude proteins were extracted by ammonium sulfate precipitation from the fermentation liquor and were studied preliminarily for fungistatic activity by different treatments. The protein was not sensitive to protease K and trypsinase and stable to heat in fungistatic activity. Its fungistasis did not change and maintained at 60% when it was treated at 40℃,60℃, 80℃ and 100℃ for 20 min or at 120℃.

关 键 词：枯草芽胞杆菌XF-1 抑菌蛋白 活性 大白菜根肿病 

分 类 号：S482.292[农业科学—农药学]