脾虚大鼠胃肠道SEC和GIP mRNA表达差异及其对胰腺外分泌的影响  

Gastrointestinal secretin and gastric inhibitory peptide mRNA expressions and their effects on pancreatic external secretion in spleen deficiency rats

作　　者：程飞[1] 刘凤华[2] 朱晓宇[3,4] 甘静宜[3,4] 王伟[2] 程桂林[2] 宋晓平[1] 许剑琴[3,4] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北京农学院动物科技系,北京昌平102206 [3]中国农业大学动物医学院,北京海淀100193 [4]农业部兽用化药和中草药创制与评价重点开放实验室,北京海淀100193

出　　处：《中国兽医杂志》2009年第7期3-6,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30771591;30771566);国家"十一五"科技支撑计划(2008BADB4B01);"中兽药添加剂的研制与开发"(2008BADB4B07)

摘　　要：以利血平大鼠脾虚模型为研究对象,采用RT-PCR技术,研究脾虚大鼠胃肠道不同组织中促胰液素和抑胃肽mR-NA表达变化及其对胰腺外分泌的影响。结果显示,与各自对照组相比,脾虚大鼠SECmRNA表达在十二指肠和回肠都显著降低(P<0.05);GIP mRNA表达在胃窦显著降低(P<0.05)。表明,SEC和GIP mRNA下调是引起脾虚的重要原因。The purpose of this study was to investigate secretin （SEC） and gastric inhibitory peptide （GIP） mRNA expressions, and effects on pancreatic external secretion in the different tissues of the gas- trointestinal tract from the reserpine-induced spleen deficiency rats using RT-PCR. The results showed that compared with their controls, the mRNA expression of SEC in the spleen deficiency rats was signifi- cantly lower （P〈0.05） in the duodenum and ileum. GIP mRNA expression decreased significantly （P〈 0.05） in the antrum. It suggested that downregulation of SEC and GIP mRNA expression is one of the critical factors contributing to spleen deficiency in rats.

关 键 词：脾虚 促胰液素 抑胃肽 RT-PCR 

分 类 号：S852.2[农业科学—基础兽医学]