玫烟色拟青霉中过氧麦角甾醇的高速逆流色谱法分离纯化及质谱分析  

Isolation and Purification of Peroxy-ergostero1 from Paecilomyces fumosoroseus by High-Speed-Counter-Current Chromatography and Identification by ESI-MS

作　　者：章能胜[1] 王金彬[1] 胡丰林[1] 汪小艳[1] 王小董[1] 李增智[1] 樊美珍[1] 

机构地区：[1]安徽省微生物防治重点实验室安徽农业大学

出　　处：《食品与发酵工业》2009年第6期14-17,共4页Food and Fermentation Industries

基　　金：国家自然科学基金项目(30570189;30871676);国家863项目(2007AA021506);安徽高校自然科学研究重点项目(DT200708)共同资助

摘　　要：采用高速逆流色谱法分离纯化玫烟色拟青霉菌丝体中的过氧麦角甾醇。溶剂系统为V(正己烷):V(乙腈):V(三氯甲烷)=5:5:1,上相为固定相,下相为流动相,转速为850r/min,流速为2mL/min。所得产物纯度经高效液相色谱分析为98.5%(峰面积归一化法),经高分辨ESI-MS质谱确认其精确分子量。High-speed-counter-current chromatography（HSCCC） was applied to the separation of peroxy-ergosterol from a mycelia strain of Paecilomyces fumosoroseus. The solvent system was n-hexane-acetonitrile-chloroform （5 ：5 ： 1 ,v/v/v） ; the upper column was solid phase and the lower column was the mobile phase with the flow rate of 2.0 mL/min and rotation speed at 850 r/min. The obtained fraction was determined by HPLC with 98.5% purity. The molecular formula of the compound was identified by ESI-MS.

关 键 词：过氧麦角甾醇 高速逆流色谱 电喷雾电离质谱 玫烟色拟青霉 

分 类 号：Q936[生物学—微生物学]