不同剂量γ射线辐照对小鼠红内期疟原虫杀伤作用的研究  

The exposure dose-effectiveness of γ irradiation on killing the plasmodium in mice red blood cells

作　　者：刘忠湘[1] 李英辉[1] 赵亚[1] 雷俊川[1] 薛采芳[1] 夏爱军[2] 张献清[2] 

机构地区：[1]第四军医大学病原生物教研室,陕西西安710032 [2]第四军医大学西京医院输血科

出　　处：《中国输血杂志》2009年第6期456-459,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探寻不影响正常红细胞活性及功能且能够有效杀伤血液中疟原虫的γ射线辐照方法。方法感染约氏疟原虫(Plasmodiumyoelii,P.y)种鼠血液,设实验组(辐照组,n=5)和对照组(未辐照,n=5),辐照组采用Gammacell 1000 Elite血液辐照仪,分别经γ射线25、35、45Gy单次均匀照射后,将所有的血液样本置于4℃存放,分别于辐照后1、3及5d取样,感染小鼠,间隔2d采血涂片镜检,计算各组小鼠原虫感染率。结果对照组小鼠出现原虫血症较早(1—2d),疟原虫感染率明显高于辐射组(3.7%vs0.07%),辐照剂量越大,血液中的原虫存活数量越少,小鼠存活时间也随之延长(8—12d);45Gy辐照剂量组4℃放置5d的血液感染小鼠,红细胞内未见疟原虫;对照组血检阳性,小鼠全部死亡。结论血液经45Gy辐照4℃放置5d,可以有效杀伤小鼠红细胞内疟原虫。Objective To study the exposure dose-effectiveness of γ irradiation on killing the plasmodium in the mice RBC, for the further exploration on the method that could kill the plasmodium in RBC without affecting the activity and function of normal RBC. Methods After infection with Plamodium yoelii （P. y）, blood was collected from mice and exposed to γ irradiation （ radiated group）. An unirradiated group served as control. In the irradiated group, P. y infected blood was divided into three aliquots, each aliquot was irradiated one time by 5, radiation using Cammacell 1000 Elite blood radiation apparatus. The dosage of each aliquot was 25, 35 and 45Gy. After irradiation, the blood samples were stored at 4℃. Then mice were inoculated with these irradiated blood stored for 1,3 or 5 days after irradiation, or with unirradiated blood. Two days later, the blood samples were taken from inoculated mice and were examined under microscope and plasmodium infection rates were calculated. Results The mice in the control group had parasitemia much earlier than those in irradiated group （ 1-2 days）, and the plasmodium infection rate in the control group was significantly higher than that in the irradiated group（3.7% vs 0.07%）. With increasing dosage of irradiation, the survived plasmodium in blood decreased, and survival of mice increased（8-12 days）. After 45 Gy irradiation and 5 day storage at 4℃, there were no plasmodium found in the red blood cell of inoculated mice. In the control group, blood testing result was positive, and all the mice died. Conclusion Plasmodium in mice RBC can be killed effectively when blood is exposed to 45Gy irradiation and stored at 4℃ for 5 days.

关 键 词：Γ射线 辐照 疟原虫 红细胞 小鼠 

分 类 号：R382.31[医药卫生—医学寄生虫学] R446.5[医药卫生—基础医学]