1株H5N1亚型高致病力禽流感病毒人工感染鸭的细胞凋亡观察  

Induction of Apoptosis by a Highly Pathogenic Avian Influenza Virus,A/duck/Guangdong/220/2004 (H5N1)in Experimentally Infected Ducks

作　　者：李玉谷[1] 叶远兰[1] 崔聪颖[1] 周泉鹤[1] 张媛[1] 马勇江[1] 李楚宣[1] 

机构地区：[1]华南农业大学兽医学院,广州510642

出　　处：《畜牧兽医学报》2009年第7期1069-1073,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省科技攻关项目(2004A2090102);广东省教育厅科学基金项目(Z02003)

摘　　要：TUNEL染色法和透射电镜观察表明,鸭静脉接种或眼-鼻-口腔-泄殖腔混合接种高致病力禽流感病毒A/duck/Guangdong/220/2004(H5N1)后,心、肝、脾、肺、肾、胰、肠、脑、胸腺和法氏囊均可发生细胞凋亡。其中,凋亡主要发生在胰腺泡上皮细胞、肾小管上皮细胞、肝细胞和肠黏膜上皮细胞;也有少量神经元、神经胶质细胞、心肌细胞、呼吸毛细管上皮细胞,胸腺、脾脏和法氏囊淋巴细胞发生凋亡。Using terminal deoxyuridine transferase nick-end labeling（TUNEL）and electron microscopy technique, the apoptosis were investigated in ducks inoculated with a highly pathogenic avian influenza virus, A/duck/Guangdong/220/2004 （HSN1） by intravenous and ocular-nasal-oral-cloacal routes. The results demonstrated that the virus-induced apoptosis occurred in the pancreas, kidney, liver, heart, lung, intestine, cerebrum, cerebellum, thymus, bursa of Fabricius and spleen, etc. The predominent apoptotic cell types were pancreatic acinar epithelial cells, renal tubular epithelial cells, hepatocytes and intestinal epithelial cells. The minority of neurons, glial cells, cardiac muscle cells, epithelial cells of lung air capillary, lymphocytes in the thymus, bursa of Fabricius and spleen also experienced apoptotic changes.

关 键 词：H5N1亚型禽流感病毒 细胞凋亡 鸭 

分 类 号：S852.659.5[农业科学—基础兽医学] S858.325.3[农业科学—兽医学]