藏鸡血管内皮生长因子基因表达与低氧适应  

Quantitative Expression of Vascular Endothelial Growth Factor Gene and Hypoxic Adaptation in Tibetan Chicken

作　　者：强巴央宗[1] 谢庄[2] 商鹏[1] 李齐发[2] 翟明霞[2] 张浩[3] 

机构地区：[1]西藏农牧学院动物科技系,林芝860000 [2]南京农业大学动物科技学院,南京210095 [3]中国农业大学动物科技学院,北京100193

出　　处：《畜牧兽医学报》2009年第7期1101-1105,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871770);国家科技支撑项目(2008BADB2B01)

摘　　要：比较藏鸡在常氧(O2,21%)和低氧(O2,13%)浓度孵化环境中,胚胎尿囊绒毛膜(CAM)组织VEGF基因mRNA表达变化及其与低地矮小隐性白鸡的差别,分析VEGF表达与低氧适应的关系。结果发现低氧刺激使藏鸡和矮小隐性白鸡CAM组织VEGF表达均上调,矮小隐性白鸡上调程度明显大于藏鸡。结果说明在低氧环境中藏鸡CAM组织VEGF表达表现一定程度的增加,有利于血管形成,表现对低氧环境的适应;而低地鸡胚胎VEGF可能表现异常表达,不利于胚胎发育和存活。In this study, the expression of VEGF gene was measured in chorioallantoic membrane （CAM） tissue of Tibetan chickens and Dwarf Recessive White chickens which were incubated at normoxia （O2,21%） and hypoxia （O2,13%）, respectively. The results showed that hypoxia-induced VEGF up-regulation occurred in CAM tissue for both breeds. However, the extent of upregulation was significantly higher in Dwarf Recessive White chickens than that in Tibetan chickens. It concluded that hypoxia induced VEGF mRNA expression to a certain degree in Tibetan, which made for angiogenesis and adaptation to hypoxic environment for Tibetan chickens. However, the VEGF expression was excess in CAM tissue in Dwarf Recessive White chickens at hypoxic condition, which made the embryo of Dwarf Recessive White chickens against to development and survival.

关 键 词：藏鸡 VEGF 低氧适应 基因表达 

分 类 号：S831.2[农业科学—畜牧学] Q344.13[农业科学—畜牧兽医]