真空、低能离子注入后HeLa细胞的吸收光谱研究  

Absorption Spectrum Study of HeLa Cells Treated with Vacuum and Low-Energy Ions Implantation

作　　者：张凤秋[1] 赵元黎[1] 葛向红[1] 刘伟[1] 张广水[1] 秦广雍[1] 

机构地区：[1]郑州大学物理工程学院,河南郑州450052

出　　处：《光谱学与光谱分析》2009年第8期2034-2037,共4页Spectroscopy and Spectral Analysis

基　　金：国家"十五"科技攻关项目(2001BA302B-03)资助

摘　　要：在选用了石蜡油作为细胞耐受真空的保护剂基础上,分别采集了人宫颈癌细胞(HeLa细胞)经真空和不同剂量低能离子注入后的紫外吸收光谱。实验结果显示:HeLa细胞在202及260nm附近均有特征性的吸收峰。数据的进一步分析发现:(1)HeLa细胞经真空处理后的紫外吸光值随着真空时间的延长而增加,且真空对细胞紫外吸收光谱的影响大于单纯的石蜡油浸泡影响;(2)真空对于HeLa细胞紫外吸光值的影响远低于低能离子注入的影响;(3)HeLa细胞在注入不同剂量的低能N+后,紫外吸光值会随着注入剂量的增大而增加。在上述分析的基础上,本文又探讨了低能N+束注入对肿瘤细胞的结构、各成分的组成比例及分子排列的影响,该研究为深入探索低能离子注入生物样品的作用机理奠定了基础。Mineral oil was selected to protect HeLa cells from water evaporation during low-energy ions implantation in the present paper. Then, HeLa cells having been treated with vacuum and low-energy N＋ ions implantation were used to collect ultraviolet absorption spectrum by spectrophotometer. Analytical results indicated that HeLa cells had some characteristic absorption peaks near 202 and 260 nm, respectively. And then the study also found： （1） The spectral intensity increased with the vacuum treatment time. In addition, the effect of vacuum on cellular spectrum was greater than that of mineral oil. （2） The influence of low energy N＋ ions on absorption spectrum was far more than that of vacuum. （3） The spectral intensity increased with the implantation dose. According to these results, the effect of low-energy N＋ ions implantation and vacuum on tumorous cells （HeLa cells）, especially on the molecular configuration and component of tumorous cells （HeLa cells） was discussed. In a word, this study provides a basis for further research on the functionary mechanism of low-energy ions implantation on biomaterial.

关 键 词：人宫颈癌细胞 真空 低能离子注入 吸收光谱 

分 类 号：O657.3[理学—分析化学]