菲及腐殖酸混合液荧光法定量分析研究  

Quantitative Analysis of Phenanthrene and Humic Acid in Solution Based on Fluorescence Method

作　　者：金丹[1] 李国刚[2] 张玉钧[1] 肖雪[1] 赵南京[1] 殷高方[1] 刘文清[1] 

机构地区：[1]中国科学院环境光学与技术重点实验室,中国科学院安徽光学精密机械研究所,安徽合肥230031 [2]中国环境监测总站,北京100029

出　　处：《光谱学与光谱分析》2009年第8期2213-2216,共4页Spectroscopy and Spectral Analysis

基　　金：国家"863"计划项目(2007AA061502)资助

摘　　要：多环芳烃作为普遍关注的优先监测污染物,在水环境中其含量很低,易受共存物腐殖酸的干扰。由于多环芳烃与腐殖酸的光谱重叠严重,很难用常规方法快速的定量检测。菲(PHE)作为多环芳烃中的模式化合物,对菲和腐殖酸(HA)的荧光光谱特性进行了研究,分析了腐殖酸共存下对菲定量检测的影响。采用平行因子算法分析混合液的三维荧光光谱,在激发波长为240～360nm(5nm为间隔)、发射波长为260～575nm(5nm为间隔)下对该体系进行了分辨研究,并对菲和腐殖酸进行荧光光谱测量。实验结果表明,此方法可用于有干扰共存下多环芳烃化合物的直接快速定量检测。Polycyclic aromatic hydrocarbons as a universal concern in the monitoring of priority pollutants have low content in the water environment and are interfered with the coexistence of humie acid. The spectra of humic acid and polycyclic aromatic hydrocarbons overlap seriously, so it was difficult to use the conventional methods for rapid quantitative detection. As a model compound of polycyclic aromatic hydrocarbons, Phenanthrene （PHE） was chosen in the experiment. The fluorescence spectra of PHE and humic acid （HA） were investigated by three dimensional fluorescence excitation-emission matrix. The method combined excitation-emission matrix fluorescence spectra with parallel factor analysis and was applied to determine PHE and HA directly. The excitation wavelength changed from 240 to 360 nm at an interval of 5 nm. The emission wavelength varied from 260 to 575 nm at an interval of 5nm. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures.

关 键 词：三维荧光光谱 荧光光谱重叠 PARAFAC算法 菲 腐殖酸 

分 类 号：X830.2[环境科学与工程—环境工程]