Premature Tapetum Degeneration: a Major Cause of Abortive Pollen Development in Photoperiod Sensitive Genic Male Sterility in Rice  

作　　者：Yinlian Shi Sha Zhao Jialing Yao 

机构地区：[1]College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

出　　处：《Journal of Integrative Plant Biology》2009年第8期774-781,共8页植物学报（英文版）

基　　金：Supported in part by the State Key Basic Research and Development Plan of China (2007CB108700);the National Key Project of Functional Genomics of Major Plants and Animals, China

摘　　要：Photoperiod-sensitive genic male-sterile （PSGMS） rice （Oryza sativa L.）, a natural mutant found in the rice cultivar Nongken 58, is very useful for the development of hybrid rice cultivars. Despite its widespread use in breeding programs, the initial stage of the abortive development of PSGMS rice and the possible cytological mechanisms of pollen abortion have not been determined. In the present study, a systematic cytological comparison of the anther development of PSGMS rice with its normal fertile counterpart is conducted. The results show that pollen abortion in PSGMS rice first occurs before the pollen mother cell （PMC） stage, and continues during the entire process of pollen development until pollen degradation. The abortive process was closely associated with the abnormal behavior of the tapetum. Although tapetum degeneration in PSGMS rice initiates already at the PMC stage, it proceeds slowly and does not complete until the breakdown of the pollen. Such cytological observations were supported by the results of the TUNEL （TdT-mediated dUTP Nick End Labeling） assay, which detects DNA fragmentation resulting from programmed cell death （PCD）, indicating that the premature tapetum degeneration is in the process of PCD.Photoperiod-sensitive genic male-sterile （PSGMS） rice （Oryza sativa L.）, a natural mutant found in the rice cultivar Nongken 58, is very useful for the development of hybrid rice cultivars. Despite its widespread use in breeding programs, the initial stage of the abortive development of PSGMS rice and the possible cytological mechanisms of pollen abortion have not been determined. In the present study, a systematic cytological comparison of the anther development of PSGMS rice with its normal fertile counterpart is conducted. The results show that pollen abortion in PSGMS rice first occurs before the pollen mother cell （PMC） stage, and continues during the entire process of pollen development until pollen degradation. The abortive process was closely associated with the abnormal behavior of the tapetum. Although tapetum degeneration in PSGMS rice initiates already at the PMC stage, it proceeds slowly and does not complete until the breakdown of the pollen. Such cytological observations were supported by the results of the TUNEL （TdT-mediated dUTP Nick End Labeling） assay, which detects DNA fragmentation resulting from programmed cell death （PCD）, indicating that the premature tapetum degeneration is in the process of PCD.

关 键 词：MALE-STERILITY Oryza sativa programmed cell death TAPETUM ULTRASTRUCTURE 

分 类 号：S511.01[农业科学—作物学] S512.103.5