平喘固本方对哮喘豚鼠肺组织β-肾上腺素能受体、腺苷酸环化酶及环磷酸腺苷的影响  

Effects of Ping Chuan Gu Ben Recipe on β-Adrenergic Receptor,Adenylate Cyclase and Cyclic Nucleotide in Pulmonary Tissue of Asthmatics Guinea Pig

作　　者：方军[1] 郭忻[1] 潘颖宜[1] 朱永健[1] 匡兴伟[1] 

机构地区：[1]上海中医药大学,上海200032

出　　处：《标记免疫分析与临床》1998年第2期73-76,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：本文介绍了平喘固本方对致敏哮喘豚鼠肺组织β－肾上腺素能受体（β－AR）、腺苷酸环化酶(AC)活性及环磷酸腺苷(cAMP)含量的影响。豚鼠随机分成四组：对照组、平喘固本方组、酮替芬（西药对照）组和固本咳喘（中药对照）组，豚鼠肺中β－AR结合容量分别为22．68±6．19、29．77±5．84、24.64±2.64和26．13±6．52fmol／mg蛋白，平喘固本方组β－AR的结合位点（Rt值）大于对照组及酮替芬组，P＜0．05。肺组织中AC活性分别为9．66±2．36、13．1±2.4、8．4±2.4和9.02±2．81pmol·min－1／mg蛋白，平喘固本方组明显高于对照组、西药组和中药组，差异显著（P＜0．05）。cAMP含量分别为0．070±0．014、0096±0．027、0．098±0．029和0．097±0．015pmol／mg组织，用药各组均高于对照组，差异显著（P＜0．05）。提示平喘固本方可以提高哮喘豚鼠脑组织中β－AR功能，激活AC，使细胞中cAMP含量增加，使致敏哮喘豚鼠症状得以改善。The effects of Ping Chuan Gu Ben Recipe on β- adrenergic receptor, adenylate cyclase and cyclic nucleotide in pulmonary tissue of asthmatic guinea pig unduced by albumin were investigated. Guinea pigs were divided into four groups: normal saline group (asthma control),Ping Chuan Gu Ben Recipe group, ketotifen group(western medicine control group) and Gu Ben Ke Chuan group(Chinese medicine control group). β-AR Rt in the lung was 22. 68 ±6. 19, 29.77±5. 84, 24. 64±2. 64 and 26. 13 ±6. 52fmol/mg of protein respectively. Rt values of Ping Chuan Gu Ben Recipe were significantly greater than those of asthma control and ketotifen group .(P<0. 05). Adenylate cyclase activities were 9. 66±2. 36, 13. 10± 2. 40, 8. 42±2. 41 and 9. 02±2. 8lpmol·min-1/mg of protein respectively. Adenylate cyclase activity of Ping Chuan Gu Ben Recipe group was significantly higher than either one of three groups (P<0.05). Cyclic nucleotide contents were 0. 070 ±0. 014, 0. 096±0. 027, 0. 098±0. 029 and 0. 097±0. 015pmol/mg tissue respectively. Contents of treated group were significantly greater than that of untreated group(P<0. 05). It is suggested that Ping Chuan Gu Ben Recipe may enhance the function of β- AR in the pulmonary tissue, thus adenylate cyclase is activated and cyclic nucleotide content is increased to alleviate the symptoms of asthmatic guinea pig.

关 键 词：哮喘 肾上腺素能 受体 腺苷酸环化酶 平喘固本方 

分 类 号：R256.120.5[医药卫生—中医内科学]