大白菜DA1基因的生物信息学解析  

Bioinformatic Analysis of DA1 Gene of Chinese Cabbage

作　　者：李利斌[1] 王殿峰[2] 刘立峰[1] 刘国明[3] 高建伟[1] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100 [2]济南市农业局,山东济南250021 [3]寿光市台头镇农业综合服务站,山东寿光262735

出　　处：《山东农业科学》2009年第7期1-3,共3页Shandong Agricultural Sciences

基　　金：国家科技基础条件平台建设项目(2005DKA21001-38);山东省农业科学院高技术自主创新基金(2007YC003)

摘　　要：运用比较基因组学的方法,首次从大白菜基因组中鉴定出一个与拟南芥中控制种子和器官大小的基因DA1同源的序列BrDA1,并对这个基因和编码蛋白的结构以及顺式反应元件进行了系统分析。结果表明,BrDA1具有LIM家族和锌指蛋白的结构特征,而且还有和泛素互作的位点,可能具有和AtDA1相似的功能。另外,本研究还发现,在大白菜和拟南芥DA1基因的启动子序列中存在多个能够响应不同激素和逆境信号的顺式反应元件。这说明,植物DA1基因不仅与种子和器官发育有关,而且与激素信号转导和逆境响应存在密切关系。本文为进一步研究DA1基因在大白菜生长发育和逆境响应中的功能以及与大白菜杂种优势的关系奠定了基础。Using the method of comparative genomics, a homolog of Arabidopsis DA1 gene controlling the seed and organ size was identified in Chinese cabbage genome, and named BrDA1. The exon - intron structure, protein domains and cis - acting elements of the gene were systematically analyzed. The results indicated that the putative protein encoded by BrDA1 was characterized with one LIM domain, one zinc -finger domain and two ubiquitin interaction motifs. It suggeted that BrDA1 might have the similar function to AtDA1 and play important roles in regulating seed and organ size. This investigation also found that there were cis -acting ele- ments existed in the promoter regions of BrDA1 and AtDA1, which could response to hormones and stress signals. The results implicated that the plant DA1 gene might not only be involved in development of seed and other organs, but also closely associated with hormone signalings and stress responses. This research laid a foundation for functional dissection of BrDA1 gene in Chinese cabbage development and stress response, and also its relationship with heterosis.

关 键 词：大白菜 DA1 结构 顺式反应元件 

分 类 号：S634.1[农业科学—蔬菜学] Q343.12[农业科学—园艺学]