盐胁迫条件下不同耐盐棉花miRNA差异表达研究  

Differential Expression of miRNA in Different Salt-tolerant Cotton Varieties under Salt Stress

作　　者：李春贺[1] 阴祖军[1] 刘玉栋[1] 沈法富[1] 

机构地区：[1]山东农业大学农学院,山东泰安271018

出　　处：《山东农业科学》2009年第7期12-17,共6页Shandong Agricultural Sciences

摘　　要：植物miRNAs在基因表达、生长发育和抵抗胁迫等方面有十分重要的作用。本试验以耐盐棉花品系山农91-11和盐敏感棉花品种鲁棉6号为试材,利用miRNA基因芯片杂交技术,分析盐胁迫条件下棉花幼苗miRNA的差异表达。结果表明:在盐胁迫条件下,共有27个miRNAs在山农91-11和鲁棉6号之间差异表达,其中山农91-11比鲁棉6号表达上调的miRNAs有11个,其中功能已知的10个分别属于miR156、miR164、miR167、miR397和miR399家族,表达下调的miRNAs有16个,其中功能已知的2个属于miR156、miR172家族。对这些miRNA家族作用的靶基因进行分析表明,这些miRNAs参与植物逆境条件下的信号传导,可能对棉花耐盐机制有重要作用。Plant miRNAs have important impacts on gene expression, growth and development, resistance to stress and so on. This study used the salt - tolerant cotton line Shannong 91 - 11 and salt - sensitive cuhivar Lumian 6 as the research materials to detect the differential expression of cotton miRNAs under salt stress by miRNA microarray. The results showed that there were 27 miRNAs expressing differently between Shannong 91 -11 and Lumian 6 under salt stress; compared with Lumian 6,11 miRNAs up -regulated their expressions, of them, 10 miRNAs whose functions had been known belonged to the miRNA families of miR156, miR164, miR167, miR397 and miR399 respectively. 16 miRNAs down- regulated their expressions, of them, 2 miRNAs whose functions had been known belonged to the miRNA families of miR156 and miR172. The analysis on the target genes of these miRNAs revealed that these miRNAs participated in the signal transduction under abiotic stress and might play important roles in salt - tolerant mechanism of cotton.

关 键 词：棉花 MIRNA芯片 盐胁迫 差异表达 

分 类 号：S562[农业科学—作物学] Q345.24[生物学—遗传学]