红藤对小鼠的免疫抑制效应  

Immunosuppressive Effect of Caulis Sargentodoxae on Mice

作　　者：王红梅 马素好 

机构地区：[1]郑州澍青医学高等专科学校,河南郑州450064

出　　处：《河南中医》2009年第8期756-758,共3页Henan Traditional Chinese Medicine

基　　金：河南省科技攻关项目(编号:0524420040)

摘　　要：目的:探讨红藤对小鼠免疫功能的影响。方法:以红藤水煎剂(25 mg/100 ml)给小鼠(18～22 g)连续灌胃7 d后,做碳廓清实验、抗体生成细胞的检测、淋巴细胞a-醋酸萘脂酶的检测、脾淋巴细胞转化实验以及迟发型变态反应(DTH)。结果:与对照组相比,红藤水煎剂能明显抑制小鼠单核-巨噬细胞的吞噬活性,降低小鼠机体抗体生成细胞分泌抗体IgM量,抑制小鼠外周血中的T淋巴细胞数目,降低脾淋巴细胞的转化率,减少DNCB致敏的小鼠两耳的重量差。经统计学处理,各实验组与对照组相比,各项指标均有极显著差异,P(0.01。结论:红藤对小鼠细胞免疫功能及体液免疫功能均有明显抑郁作用,为今后中药免疫抑制剂的开发提供了科学的依据。Objective：To probe the immunosuppressive effect of caulis sargentodoxae on mice.Methods：Consistent gastric perfusion of caulis sargentodoxae 25mg/100ml were givern to mice（18-22g）for 7days to detect the carbon clearance,antibody producing cells,lymphocyte a-naphtyl acetate esterase,transformation of spleen lymphocyte,delayed-type hypersensitivity.Results：Compared with control group,caulis sargentodoxae decoction inhibited the activity of mononuclear-macrophage,and decreased the antibody-secreted IgM content of antibody producing cells,inhibited the number of periphearal blood T lymphocyte,decreased the trasformation rate of spleen lymphocyte,decreased the weight difference of DNCB-sensitized mice ears.Compared with those of control group,all indexes of experiment groups had significantly statistic difference（P〈0.01）.Conclusion：Caulis sargentodoxae has significant inhibiting effects on the immunofunction of cells and body fluids,which provides a scientific hasis for the development of Chinese medicinal immunosuppressant.

关 键 词：红藤 免疫抑制 单核-巨噬细胞 淋巴细胞 IGM 小鼠 

分 类 号：F285.5[经济管理—国民经济]