新生期大鼠皮下注射谷氨酸单钠后下丘脑-垂体-肾上腺-胸腺轴的改变  

Changes of hypothalamic-pituitary-adrenal-thymus (HPAT) functional axis in rats after neonatal treatment with monosodium glutamate

作　　者：蔡定芳[1] 刘彦芳[1] 

机构地区：[1]上海医科大学华山医院

出　　处：《中国病理生理杂志》1998年第4期342-326,共1页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金;卫生部科学研究基金;上海市科学技术发展基金;上海市卫生系统"百人计划"基金

摘　　要：目的：观察弓状核（ＡＲＣ）毁坏后下丘脑－垂体－肾上腺－胸腺轴（ＨＰＡＴ）的改变。方法：大鼠出生后第２、４、６、８、１０ｄ皮下注射谷氨酸单钠（ＭＳＧ）４ｍｇ／ｇ，ｂ．ｗ。成年后检测ＨＰＡＴ轴的形态与功能。结果：ＭＳＧ大鼠体重明显轻于生理盐水组，每１００ｇ体重所含垂体重量高于生理盐水组，肾上腺与胸腺重量未见明显差异；室旁核（ＰＶＮ）促肾上腺皮质激素释放激素阳性细胞、正中隆起（ＭＥ）促肾上腺皮质激素释放激素阳性纤维及垂体前叶促肾上腺皮质激素分泌细胞数量明显多于生理盐水组，染色较深；血浆皮质酮及血浆促肾上腺皮质激素浓度高于生理盐水组。胸腺萎缩，淋巴细胞增殖反应、自然杀伤细胞细胞毒活性及白细胞介素２、γ干扰素的诱生能力明显低于生理盐水组。结论：１ＭＳＧ－大鼠ＨＰＡ轴功能亢进；２ＭＳＧ－大鼠细胞免疫受抑。AIM:To observe the changes of HPAT axis with lesion of arcuate nucleusMETHODS:Newborn Sprahue-Dawley rats were given subcutaneous injection of 4mg/g of monosodium glutamate (MSG) on days 2,4,6,8,10 of postnatal life, after they were adult, the morphological and functional changes of HPAT axis were examined. RESULTS:MSG-rats showed an increase of the weight of pituitary per 100gram body weight, while there were no difference in the weights of adrenal and thymus; the number of corticotropin releasing hormone (CRH) immunoreactive (IR) positive cells in paraventricular nucleus , CRH IR positive nerve teminals in ME and ACTH IR positive cells in pituitary increased significantly; baseline of plasma ACTH and corticosterone rose. Thymus atrophied with lymphocytes showing proliferative reactivity with natural killer cell cytotoxicity activity, productivity of IL-2 and interferon-γ declines significantly.CONCLUSION: MSG-rats showed hypofunction of HPA axis and the cellular immunofunction was inhibited.

关 键 词：谷氨酸盐类 弓状核 神经内分泌 免疫调节 HPAT 

分 类 号：R335[医药卫生—人体生理学] Q45[医药卫生—基础医学]