中缝隐核至导水管腹侧部途径的儿茶酚胺中间神经元定位分析  

DISTRIBUTION OF CATECHOLAMINE NEURONS IN THE PATHWAY FROMNUCLEUS RAPHE OBSCURUS TO VENTRAL PERIAQUEDUCTAL GREY

作　　者：卢宁[1,2] 周新妹[1,2] 龚茜玲[1,2] 

机构地区：[1]上海医科大学基础医学院生理学教研室 [2]浙江省嘉兴卫生学校

出　　处：《上海医科大学学报》1998年第4期273-276,共4页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金

摘　　要：研究脑内交感抑制系统中中缝隐核（ＮＲＯ）至中脑导水管周围灰质腹侧部（ｖＰＡＧ）多突触通路的儿茶酚胺中间神经元定位。方法在戊巴比妥钠麻醉的大鼠，应用原位杂交组织化学法检测电刺激激活ＮＲＯ对酪氨酸羟化酶（ＴＨ）ｍＲＮＡ表达的影响，同时采用辣根过氧化物酶（ＨＲＰ）逆行标记与免疫组化双标法以观察投射至ｖＰＡＧ的脑干儿茶酚胺能神经元分布。结果激活ＮＲＯ１８～２０ｈ后引起脑干延髓头端腹外侧区（ｒＶＬＭ）、孤束核（ＮＴＳ）、蓝斑（ＬＣ）、中缝背核的ＴＨｍＲＮＡ的表达增加；ＨＲＰ／ＴＨ双标细胞分布于ｒＶＬＭ（Ｃ１）、ＮＴＳ、ＬＣ、黑质网带、室周灰质等区。结论ＮＲＯ至ｖＰＡＧ途径的肾上腺素、去甲肾上腺素神经元主要位于ｒＶＬＭ的Ｃ１区、ＮＴＳ、ＬＣ。おURPOSE To examine the distribution of catecholamine neurons in the pathway from nucleus raphe obscurus (NRO) to the ventral periaqueductal grey (vPAG). To study the expression of tyrosine hydroxylase (TH) mRNA actvated by stimulation of NRO and the distribution of catecholamine neurons projecting to vPAG.METHODS Experiments were performed in rats anaesthetized with sodium pentobarbital. Perfusion was performed 18～20 hours after stimulating NRO for 1 hour. The brainstem sections were then prepared for visua￣lization of TH mRNA positive cells with in situ hybridization. Furthermore, after iontophoretic application of HRP into vPAG, the brainstem sections were processed for visualization of retrogradely labeled cells and immu￣nodetection of TH with ABC-DAB immunohistochemical techniqne.RESULTS It was observed that stimulation of NRO resulted in an increase in expression of TH mRNA in C1 of rostral ventrolateral medulla(rVLM), nucleus tractus solitarius(NTS), locus ceruleus(LC) and dorsal raphe(DR). Double-labeled neurons were observed in C1 of rVLM, NTS, LC, substantia nigra reticularis (SNR) and periventricular grey.CONCLUSIONS The results reveal that the cell bodies of adrenalinergic and noradrenalinergic neurons projecting to vPAG from NRO are located mainly in C1 of rVLM, NTS and LC.

关 键 词：中缝隐核 VPAG 儿茶酚胺 神经元 

分 类 号：R338.1[医药卫生—人体生理学]