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机构地区:[1]中国预防医学科学院寄生虫病研究所 [2]南京药物研究所
出 处:《中国寄生虫学与寄生虫病杂志》1998年第3期189-192,共4页Chinese Journal of Parasitology and Parasitic Diseases
基 金:国家自然科学基金
摘 要:目的探测进入抗氯喹疟原虫中的氯喹量较敏感株低的原因是受阻于感染的红细胞,还是疟原虫自身,以及抗氯喹疟原虫是否具有快速排出氯喹的特点。方法:给两株感染小鼠ig氯喹,用高压液相(外标法)检测感染红细胞和疟原虫中的氯喹量。结果:ig氯喹4.06mg/kg与400mg/kg氯喹3h后,敏感株感染红细胞和抗氯喹株感染红细胞中的氯喹量无明显差异,而抗氯喹疟原虫中的氯喹量,两个剂量组分别较敏感的低54.0%与42.1%(P<0.001)。比较ig氯喹3h与7h后疟原虫积聚的氯喹量,敏感株两者相同,抗性株7h后不仅无明显减少,相反地上升(0.452±0.079nmol/mg蛋白与0.559±0.124nmol/mg)蛋白(P<0.02)。结论:进入抗氯喹疟原虫中的氯喹量低,其受阻部位是在疟原虫本身而不是在感染的红细胞,未发现抗氯喹疟原虫快速排出氯喹。AIM:To explore whether the difference in the amount of chloroquine (CQ) accumulated in CQ resistant (CR) and in CQ sensitive (CS) P.berghei is involved in the infected erythrocytes (RBC) or in the parasites. METHODS:The concentration of CQ in infected RBC and parasites following ig administration of CQ to CS and CR infected mice was determined by HPLC method (extra standard). RESULTS:3 h after 4.06 mg/kg and 400 mg/kg administration,the accumulation of CQ in both infected RBC was similar,but in CR was 54.0% and 42.1% less than in CS ( P <0.001) respectively.The CQ accumulation in CS 3 h and 7 h after ig administration of CQ 400 mg/kg was similar (0.780±0.128 nmol/protein and 0.760±0.180 nmol/mg protern),while in CR the CQ accumulation was higher at 7 h than that at 3 h after CQ administration (0.452±0.079 nmol/mg protein and 0.559±0.124 nmol/mg protein P <0.02). CONCLUSION:The low CQ accumulation in CR is attributable to the parasite rather than to the infected RBC,and CR was not found to excrete CQ more rapidly than CS.
关 键 词:疟原虫 伯氏疟原虫 氯喹 积聚 排出 药敏性 抗性
分 类 号:R382.31[医药卫生—医学寄生虫学] R978.61[医药卫生—基础医学]
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