辛伐他汀抑制鼻咽癌细胞增殖和亲环素A表达  

Inhibition of Proliferation and Cyclophilin A Expression in Nasopharyngeal Carcinoma Cells by Simvastatin

作　　者：涂永生[1] 王红艳[2] 李建华[1] 彭妙茹[1] 

机构地区：[1]广州医学院生理学教研室,广东广州510182 [2]广州医学院病理学教研室,广东广州510182

出　　处：《临床医学工程》2009年第8期1-3,共3页Clinical Medicine & Engineering

基　　金：广东省医学科研基金项目(项目编号:B2006074);广州医学院科学研究项目(项目编号:04-K-15)

摘　　要：目的研究辛伐他汀对鼻咽癌细胞株CNE2细胞增殖和细胞周期的作用以及对亲环素A表达的影响。方法用CCK-8方法观察辛伐他汀对鼻咽癌细胞株CNE2细胞增殖的抑制作用,用流式细胞仪检测辛伐他汀对鼻咽癌细胞株CNE2细胞周期分布的影响,用蛋白免疫印迹检测亲环素A蛋白表达。结果CCK-8方法结果显示,辛伐他汀抑制鼻咽癌细胞株CNE2细胞增殖,作用72h时IC50为2.0μmol/L。流式细胞仪结果表明辛伐他汀将鼻咽癌细胞株CNE2细胞阻滞在细胞周期的G0/G1期,蛋白免疫印迹结果显示辛伐他汀明显抑制亲环素A蛋白表达,以上效应均呈浓度依赖性。结论辛伐他汀能浓度依赖性地抑制CNE2细胞增殖,将细胞阻滞在G0/G1期,同时伴随有亲环素A表达减少。Objective To examine the effect of simvastatin on nasopharyngeal carcinoma cell line CNE2 cells proliferation, cell cycle progression and eyclophilin A expression in vitro. Methods CCK-8 assay was employed to determine proliferation inhibition ofsimvastatin on CNE2 cells. Flow cytometry was used to assess the effect of simvastatin on cell cycle distribution. Western blot was applied to detect the cyclophilin A expression. Results Simvastatin does-dependently inhibited proliferation ofnasopharyngeal carcinoma CNE2 cells with IC 50 value of 2μmol/L for 72 h by CCK-8 assay. The result of flow cytometry and western blot demonstrated that simvastatin was able to induce G0/G1 cell-cycle arrest and decreased expression of cyclophilin A of CNE2 cells in a dose-dependent way. Conclusion These results proved that simvastatin induced CNE2 cells proliferation inhibition and cell cycle arrest at G0/G1 phase in vitro, which was associated with decreased expression ofcyclophilin A.

关 键 词：亲环素A 辛伐他汀 鼻咽癌 增殖 

分 类 号：R965.1[医药卫生—药理学]