血清乙醇脱氢酶活性测定及其临床意义  

An assay for alcohol dehydrogenase activity in serum and its clinical significance

作　　者：卓孝福[1] 陈仁奋[2] 郑靖[2] 

机构地区：[1]福建医科大学检验系,福州350004 [2]福建医科大学附属第一医院检验科

出　　处：《上海医学检验杂志》1998年第2期84-86,共3页Shanghai Journal of Medical Laboratory Sciences

摘　　要：本文建立一种用自动生化分析仪双试剂法检测血清乙醇脱氢酶（ADH）活性的方法。同时对100例肝功正常者与9例原发性肝癌、10例肝硬化、40例肝炎患者测定血清该酶活性。在反应体系中，NAD+和乙醇浓度分别为10．0mmol／L和25．0mmol／L，缓冲液为pH9．00的0．1mol／L甘氨酸-NaOH溶液。ADH对NAD+和乙醇的Km值分别为1．67mmol／L和3．03mmol／L。批内CV为0．7％～5.4％。肝功正常者血清ADH活性为1．4±1．2U／L。原发性肝癌为11．4±2．5U／L，肝硬化为4．6±1．5U／L和肝炎为5．5±1．6U／L。这些患者血清ADH活性显著高于肝功正常者。原发性肝癌患者血清ADH活性显著高于肝硬化和肝炎患者。这种简便、准确、灵敏的测定ADH活性方法可用于临床常规分析。It was described an improved method for determination of alochol dehydrogenase (EC 1. 1. 1. 1 ) activityfor Chinese by automatic analyzer, based on conversion of ethanol to acetaldehyde with simultaneous reduction of NAD+ in glycine-NaOH buffer (pH 9.0) at 37℃. The final concentration of NAD+ was 10.0 mmol/L and ethanol 25. 0 mmol/L. The Km for NAD+ in this assay was 1, 67 mmol/L and ethanol 3.03 mmo1/L-Within precisi0n (CV) was from 0. 7% to 5. 4% over the range of alcohol dehydrogenase activity from 1. 3to 30- 0 U/L. The mean activity of alcohol dehydrogenase in healthy controls (1. 4t 1. 2 U/L) was significantly lower (P<0.01) than the mean values in primary hepatic carcinoma (11. 4± 2. 5 U/L), liver cirrhosis (4. 6±1. 5 U/L), and hepatitis (5. 5±1. 6 U/L). The activity in primary hepatic carcinoma was significantly higher (P<0.01) than in liver cirrhosis and hepatitis. Because of its simplicity, accuracy, sensitivity,and requirement of small sample volume, we consider the method suitable for routine use in clinical laboratorles.

关 键 词：乙醇脱氢酶 血清 自动生化分析 

分 类 号：R446.112[医药卫生—诊断学]