套式聚合酶链反应法检测军团菌  

Detection of Legionella DNA By Nested Polymerase Chain Reaction

作　　者：郭醒华 李月久[1,2,3] 郭军巧 金抗[1,2,3] 

机构地区：[1]辽宁省卫生防疫站 [2]中国医科大学公共卫生学院 [3]中国医科大学附属第一医院

出　　处：《中国公共卫生》1998年第8期490-491,共2页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金

摘　　要：应用套式聚合酶链反应（ｎｅｓｔｅｄ－ＰＣＲ）技术，建立了扩增嗜肺军团菌巨噬细胞感染增强因子（ｍｉｐ）基因序列的方法。对１４株标准军团菌和５株标准大肠埃希氏菌等进行检测。结果仅嗜肺军团菌扩增出６４９ｂｐ特异性片段，扩增灵敏度为１００ＣＦＵ／ｍｌ。对１７份人血标本进行检测。The nested polymerase Chan reactiion (nestedPCR)has been adopted to amplily the specific sequences of the macrophage infectivity potentiator(mip)gene of Legionella pneumophila(Lp).14 strains of Lp and 5 strains of non Legionella were tested.The results shown that only Lp amplified products had 649 bp specific DNA fragment.The sensitivity of amplification was about 100CFU/ml DAN of Lp.It was used to detect Legionella DNA in 17 blood specimens (including 6 specimens collected from patients with Legionnaires′ disease).It was shown that the sensitivity of nestedPCK was significantly higher than that of serological and bacteriological culture method.The specificity were 100%,too.The above results suggest that nestedPCR may be a specific,sensititive and rapid technique for the diagnosis of Legionnaires disease

关 键 词：军团菌 聚合酶链反应 套式 诊断 

分 类 号：R446.5[医药卫生—诊断学] R378.99[医药卫生—临床医学]