草鱼Annexin A4基因克隆、表达特性及进化  

Cloning,Tissue-Specific Expression and Evolutional Analysis of Grass Carp Annexin A4 Gene

作　　者：吴初新[1] 马梅生[1] 王丽坤[1] 胡成钰[1] 

机构地区：[1]南昌大学生命科学与食品工程学院,南昌330031

出　　处：《动物学杂志》2009年第4期8-13,共6页Chinese Journal of Zoology

基　　金：江西省重点科技攻关项目(No.20061B0260301);江西省教育厅项目(GJJ09057);江西省研究生创新专项资金项目(YC08A018)

摘　　要：草鱼(Ctenopharyngodon idellus)Annexin A4基因克隆于肝肾cDNA文库,该cDNA全长为1307bp,其中,5′非编码区为104bp,3′非编码区为237bp,开放阅读框为966bp,编码321个氨基酸。草鱼Annexin A4编码的蛋白质N端由15个氨基酸残基组成,C端具有4个重复序列,每个重复序列都有一个Ⅱ型钙结合位点,在第4个钙结合位点中包含一个"KGD"序列。Annexin A4在草鱼肝、肾、脾、心、鳃、肠中的表达量均较高,而肌肉和脑中不表达;PolyⅠ:C诱导后,其表达均有所下调。系统发育树显示,草鱼Annexin A4与斑马鱼(Danio rerio)的进化距离最近。适应性进化分析没有检测到正选择位点,表明Annexin A4非常保守,受到强烈的功能约束。The Annexin A4 gene was cloned from the Grass Carp （Ctenopharyngodon idellus ） liver and kidney cDNA library. The full sequence was 1 307 bp, containing a 5＇ untranslated region of 104 bp and a 3＇ untranslated region of 237 bp. The open reading frame was 966 bp which could code a 321 amino acids peptide. The Grass Crap Annexin A4 had a tail （N-terminal region） and a core domain （C-terminal region）. The N-terminus was composed of 15 residues. The core domain was made up of four similar repeats, each covering a type Ⅱ calcium binding site. And there was a KGD motif in the fourth site. The Grass Crap Annexin A4 had a high expression level in the liver, kidney, spleen, heart, gill and intestine, but its expression was not detected in the muscle and brain. Additionally, down-regulated expression of Annexin A4 was detected in all of the six tissues after Grass Carp was challenged by Poly Ⅰ： C. Phylogenetic tree analysis revealed that Grass Carp Annexin A4 shared the highest homology with that of Zebra Fish （Danio rerio ）. Positive selection sites were not detected by adaptive evolutional analysis, and therefore Annexin A4 might be highly conserved and have strong functional constraint.

关 键 词：膜联蛋白A4 表达 适应性进化 POLY Ⅰ:C 草鱼 

分 类 号：S917.4[农业科学—水产科学]