基于ISSR标记和线粒体Cytb基因分析高原鼠兔的遗传多样性及其遗传分化  

Genetic Diversity and Differentiation of Ochotona curzoniae Based on ISSR and Cyt b Gene

作　　者：葛艳丽[1,2] 林恭华[1,2] 慈海鑫[1,2] 张同作[1] 唐利洲[1,2] 苏建平[1] 

机构地区：[1]中国科学院高原生物适应与进化重点实验室,中国科学院西北高原生物研究所,西宁810001 [2]中国科学院研究生院,北京100049

出　　处：《动物学杂志》2009年第4期34-40,共7页Chinese Journal of Zoology

基　　金：中国科学院知识创新工程领域前沿项目(No.CXLY-2002-3)

摘　　要：高原鼠兔(Ochotona curzoniae)是青藏高原的特有动物和关键种。本实验应用ISSR分子标记和细胞色素b(Cytochrome b,Cytb)基因序列分析了雅鲁藏布江两岸高原鼠兔4个种群的遗传多样性和遗传分化。结果显示,两种标记所得到的4个种群的遗传多样性都较高,并以Cytb基因为指标的遗传多样性水平在种群间体现出较大差异。在ISSR标记中,分子方差分析(AMOVA)表明种群间的遗传变异为13.50%,种群分化较低且UPGMA聚类时江北岸与南岸的种群有交叉;而在Cytb基因中遗传变异主要发生在种群间,占79.24%,种群间存在着显著的遗传分化,且江北岸和南岸的两个种群分别聚为一类。研究结果表明,mtDNA基因在反映种群遗传多样性和遗传分化上较ISSR分子标记相对具有优势。The Plateau Pika （Ochotona curzoniae） is endemic to the Qinghai-Tibetan Plateau and is a key species in the alpine meadow ecosystem. In this paper, genetic diversity and differentiation of four populations of Plateau Pika from both banks of the Brahmaputra River were assessed with inter-simple sequence repeat （ISSR） and Cyt b gene sequences. Our results showed that the genetic diversity of Plateau Pika was relatively high and there were much larger deviations among populations based on Cyt b gene than those based on ISSR marker. Analysis of molecular variance （AMOVA） in ISSR revealed a low level of genetic differentiation among the four populations. Of the total genetic variance, 13.50% was attributable to among-population variance, and the results of the UPGMA cluster analysis indicated that populations from north bank and south bank of the Brahmaputra River were intersectant. However, AMOVA in Cyt b gene indicated that 79.24 % of the total genetic variance was explained by differences among the four populations, and the UPGMA cluster of the four populations demonstrated that the populations from north bank and south bank were clustered into two single groups, respectively. Therefore, we suggest that Cyt b gene is a better marker than ISSR in reflecting the genetic diversity and genetic differentiation of populations.

关 键 词：高原鼠兔 ISSR 细胞色素B基因 遗传多样性 遗传分化 

分 类 号：Q953[生物学—动物学]