兔胚胎时期特异性基因相关新片段的克隆  

Cloning of Stage-specific Gene Fragments in Rabbit Embryos

作　　者：齐冰[1] 常正尧[1] 李效良[1] 李永芳[1] 陈清轩[2] 李文雍[3] 

机构地区：[1]泰山医学院生物科学系,泰安271016 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]阜阳师范学院生命科学学院,安徽阜阳236041

出　　处：《动物学杂志》2009年第4期41-46,共6页Chinese Journal of Zoology

基　　金：泰山医学院博士科研启动基金项目;国家自然科学基金项目(No.30871415)

摘　　要：阶段特异性基因的表达是早期胚胎发育过程中的重要事件,对植入前胚胎基因表达模式的研究是进一步研究植入前胚胎发育调控机制的前提。本实验利用mRNA差异显示技术来研究兔(Oryctolagus cuniculus domestica)植入前各期胚胎的基因表达差异。在获得的42个阳性阶段特异性表达的基因中,有5个在NCBI和EMBL数据库中没有同源序列,登录EMBL,申请了登录号。这些新基因片段都是桑葚期特异表达的基因,而且在以后的囊胚期也有表达。兔由母源型调控向合子型调控的过渡是在8～16细胞期开始的,在桑葚期开始表达的这些基因片段应该是兔胚胎时期特异性基因。这些基因的克隆将为进一步研究兔的植入前胚胎发育模式奠定基础。Proper expression of stage-specific genes is very important for early embryo development. The mRNA differential display was used to study the stage-specific genes in rabbit preimplantation embryos. A total of 42 positive differential fragments were obtained, of which 5 fragments had no homolog in the NCBI and EMBL. These fragments were submitted to the EMBL and accession numbers were given. All these 5 genes were specifically expressed in morula stage. Maternal-to-zygotic transition of rabbit starts from 8- 16 cell stage, and therefore, these genes should be embryo-specific. This research would lay a foundation for further investigating the transcriptional modulation of rabbit preimplantation embryo development.

关 键 词：兔 MRNA差异显示技术 桑葚胚 合子型基因 

分 类 号：S829.1[农业科学—畜牧学] Q78[农业科学—畜牧兽医]