CREB在拟黑多刺蚁不同品级脑部mRNA水平的定位  

Distribution of CREB mRNA in the Brain of Polyrhachis vicina

作　　者：李青[1] 何慧[1] 罗晶[1] 张文娜[1] 奚耕思[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《动物学杂志》2009年第4期89-95,共7页Chinese Journal of Zoology

基　　金：陕西省农业攻关项目(No.2008K02-10)

摘　　要：为探讨CREB在拟黑多刺蚁(Polyrhachis vicina)不同品级脑部mRNA水平的表达,采用地高辛标记法原位杂交技术对拟黑多刺蚁工蚁、雌蚁、雄蚁3个品级脑部CREB mRNA的表达进行了定位研究。结果显示,CREB mRNA在拟黑多刺蚁不同品级脑部均有广泛表达。阳性反应主要分布在蕈形体冠部的Kenyon细胞、视叶和嗅叶等部位。在3个不同品级蚂蚁的脑部中,工蚁的嗅球和蕈形体内有较明显的CREB mRNA阳性反应,雄蚁的视叶具有较强的阳性反应,与工蚁和雄蚁相比,雌蚁脑部各个部位的阳性表达都较弱。推断CREB可能在视觉和嗅觉信息的获取与整合中起着重要作用,且与不同品级蚂蚁的行为相关。The distribution of CREB mRNA was investigated by in situ hybridization in the brain of three castes of Polyrhachis vicina. Extensive expression of CREB mRNA was observed in the brain of all three castes. The prominent positive reaction was observed in the Keyron cells of mushroom body, the optic lobe and the olfactory lobe. Relatively higher expression was found in the olfactory lobe and mushroom body of workers＇ brain as well as in the optic lobe of males＇ brain. These results suggest that CREB may play a critical role in obtaining and integrating the visual and olfactory information in the nervous svstem, and mav be relevant with social community behaviors.

关 键 词：拟黑多刺蚁 CREB 原位杂交 

分 类 号：Q963[生物学—昆虫学]