自体外周血干细胞移植后血小板恢复的预测指标  

The predictor for platelet recovery after PBSCT

作　　者：冯茹[1,2] 岛崎千寻[1,2] 稻叶亨 周淑芸[1,2] 

机构地区：[1]第一军医大学南方医院血液科 [2]日本京都府立医科大学第二内科

出　　处：《中华血液学杂志》1998年第8期413-417,共5页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：目的：寻找更确切、有效、方便的预测自体外周血干细胞移植（ＡＰＢＳＣＴ）后血小板恢复能力的指标。方法：利用巨核细胞集落形成单位（ＣＦＵＭＫ）、流式细胞术等技术检测１７例恶性血液病及实体瘤患者外周血干细胞移植物中巨核细胞前体细胞的数量。结果：①ＣＤ３４＋细胞中同时表达血小板膜糖蛋白者＜５％；②ＣＤ３４＋／ＣＤ４１ａ＋细胞的数量与ＡＰＢＳＣＴ后血小板恢复时间之间的相关性较ＣＤ３４＋细胞数量与血小板恢复时间之间的相关性好；③ＣＦＵＭＫ的数量与血小板恢复之间有较好的相关性；④ＣＤ３４＋／ＣＤ４１ａ＋细胞数量与ＣＦＵＭＫ数量之间具有较好的相关性。结论：移植物中ＣＤ３４＋／ＣＤ４１ａ＋细胞数量及ＣＦＵＭＫ数量均可作为ＡＰＢＳＣＴ后血小板恢复的预测指标，但以前者更方便。Objective:In order to find out a predictor more accurate, reliable and convenient for platelet recovery capacity after peripheral blood stem cell transplantation (PBSCT). Methods: The number of megakaryocyte progenitor cells in PBSC grafts of 17 patients with hematological malignancies or solid tumors was measured by clonogenic (CFUMK) and flow cytometric assays. Results:①The expression of platelet membrane glycoprotein was on <5% of CD34+ cells. ②The time needed for platelet recovery was better correlated with the number of CD34+/CD41a+ cells than with the total number of CD34+ cells. ③There was a close correlation between the number of CFUMK and the time needed for platelet recovery. ④Correlation was observed between the number of CD34+/CD41a+ cells and that of CFUMK. Conclusion:The numbers of CD34+/CD41a+ cells and CFUMK are predictors for platelet recovery after PBSCT, but the former may be a more convenient and reliable marker for clinical study.

关 键 词：干细胞移植 外周血 血小板 CFU-MK 

分 类 号：R457[医药卫生—治疗学]