槐耳在实验性肝癌中对肝脏间质细胞的影响  

The Effect of PS-T in Experimental Hepatic Carcinoma

作　　者：陆鹏[1] 陈莉[1] 

机构地区：[1]南通大学医学院病理教研室,江苏南通226001

出　　处：《辽宁中医药大学学报》2009年第8期229-232,共4页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:通过在制备动物实验性肝癌的过程中给予槐耳清膏干预,观察并比较这种干预作用的效果及其对白细胞介素-2受体(IL-2R)阳性细胞(interleukin-2 receptor positive cell)、枯否细胞(Kupffer cell)、肝脏NK细胞(hepatic natural killer cell)的影响。方法:应用二乙基亚硝胺(N-nitrosodiethylamine,DENA)制备大鼠肝癌模型,并在制备过程的不同阶段灌胃槐耳清膏干预。于实验第20周处死动物,进行病理学检查,观察各组不典型增生与癌变率,免疫组化法检测IL-2R、巨噬细胞标志物CD68、NK细胞标志物CD57。用图像分析技术确定IL-2R阳性细胞、巨噬细胞、NK细胞的数量。并对各例肝癌中的这些细胞数量进行相关分析。结果:槐耳清膏干预组及对照组中IL-2R阳性细胞数量的差异具有显著性(P<0.05),IL-2R阳性细胞数量在对照组中明显低于槐耳清膏干预组(P<0.05);CD68阳性细胞数在对照组中明显低于槐耳清膏干预组(P<0.05),CD57阳性细胞数的差异无显著性;CD68、CD57阳性细胞数呈线性正相关。结论:槐耳清膏能有效增加肝癌组织中IL-2R阳性细胞、枯否细胞和肝脏NK细胞的数量,可能对肝癌的形成和发展有一定的抑止作用。Objective ： Interfere the process of animal experimental hepatic carcinoma preparation by PS-T. Observe and compare the effect of the interfering to the expression of interleukin-2 receptor（IL-2R） positive cells, Kupffer cell and NK cell.Methods ： Apply N-nitrosodiethylamine（DENA） to prepare the rat hepatic carcinoma model. Pour PS-T into the stomach in different phases of the preparation. Kill all the rats in week 20. Do the pathological examine to observe the atypical proliferation and carcinoma forming rate. Determine the IL-2R positive cells, Kupffer cell and NK cell in hepatic tissue by immunohistochemistry, and take count of IL-2R positive ceils, Kupffer ceil and NK cell by using computer image analyzing system. Results ： By applying PS-T, the experimental hepatic carcinoma forming rate could be decreased, and IL- 2R positive cells, Kupffer cell and NK cell could increased significantly（P〈0.05）.Conlusions ： PS-T could significantly restrain the forming of hepatic carcinoma by increasing the amount of IL-2R positive ceils Kupffer cell and NK ceil in liver.

关 键 词：IL-2R阳性细胞 枯否细胞 肝脏NK细胞 肝癌 免疫组化 

分 类 号：R285.5[医药卫生—中药学]