HBV感染者外周血PBMC中TLR4 mRNA与病毒载量的相关性研究  

Correlation between the TLR4 mRNA levels in peripheral blood mononuclear cells and HBV DNA in hepatitis B-virus infected subject

作　　者：李春江[1] 孙蕾[2] 张敬凯[3] 马淑霞[4] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]佳木斯市中心医院B超室,黑龙江佳木斯154001 [3]鸡西市矿业集团总医院检验科,黑龙江鸡西158001 [4]佳木斯大学儿童神经康复实验室,黑龙江佳木斯154007

出　　处：《中国微生态学杂志》2009年第7期633-635,共3页Chinese Journal of Microecology

摘　　要：目的探讨乙型肝炎病毒感染者外周血PBMC中TLR mRNA与乙肝病毒复制的相关性。方法采用逆转录PCR检测外周血单个核细胞(PBMC)中TLR4 mRNA的含量,实时荧光定量Real Time PCR的方法检测HBV DNA,进行相关性分析。结果不同病毒载量组(<1×103copies/μg DNA,1×103copies/μg DNA<且<1×105copies/μg DNA,>1×105copies/μg DNA)TLR4 mRNA水平差异具有显著性(P<0.01),HBV病毒载量的对数值与TLR4 mRNA的含量存在负相关(r=-0.537,P<0.01)。TLR4 mRNA的相对表达量与患者的ALT、AST呈正相关(r=0.608、r=0.659,P<0.01)。结论HBV在患者体内复制活跃、病毒载量增高与外周血单个核细胞TLR4mRNA的表达下调有关。Objective To investigate the correlation between the TLR4 mRNA levels in peripheral blood mononuclear cells（PBMC） and hepatitis B virus DNA from HBV infected patients. Method TLR4 mRNA levels in PBMC was measured by RT-PCR, and the HBV DNA viral load of hepatitis B virus infected patients was measured by using real time fluorescence quantitative PCR. The correlation between the TLR4 mRNA levels and HBV DNA of the patients was analyzed. Result TLR4 mRNA level in three groups with different viral load（ 〈 1 × 10^3copies/μg DNA; 1 × 103copies/μg DNA 〈 and 〈 1 × 105eopies/μg DNA; 〉 1 × 105copies/μg DNA） were significantly different （ P 〈 0. 01 ）. The log values of HBV load in all patients had negative correlation with the TLR4 mRNA levels in their PBMCs （r = -0.537 ,P 〈 0.01 ）. Meanwhile the TLR4 mRNA levels had positive correlation with ALT and ＆ST（ r = 0.608 ,r = 0. 659 ,P 〈 0.01 ）. Conclusion The active replication of HBV DNA and higher level of HBV DNA copies in hepatitis B virus infected suggested that they have relation with the lower expression of TLR4 mRNA in PBMC.

关 键 词：乙型肝炎病毒 病毒载量 TLR4mRNA 实时定量PCR 

分 类 号：R512.62[医药卫生—内科学]