套式PCR检测蚊体内疟原虫的敏感性与特异性  

SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION IN DETECTING PLASMODIUM VIVAX IN INFECTED MOSQUITOES

作　　者：李凤舞[1,2] 牛春[1,2] 叶炳辉 诸欣平[1,2] 陈佩惠[1,2] 

机构地区：[1]首都医科大学寄生虫学教研室 [2]南京医科大学寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》1998年第3期164-167,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的：分析套式聚合酶链反应技术检测蚊体内疟原虫的敏感性与特异性。方法：采用２对针对间日疟原虫小亚单位核糖体核糖核酸基因（ＳＳＵｒＤＮＡ）的特异引物，利用套式ＰＣＲ技术，从蚊体ＤＮＡ样本中，扩增间日疟原虫ＳＳＵｒＤＮＡ片段，进行间日疟原虫的检测。结果：扩增产物经琼脂糖凝胶电泳分析，可见扩增出特异的约１２１ｂｐ大小的ＤＮＡ片段，估测每份蚊虫ＤＮＡ样本中含有３个以上子孢子或１００只蚊中含有１只阳性蚊即可得此片段，而对恶性疟原虫、食蟹猴疟原虫、约氏疟原虫及正常蚊虫ＤＮＡ不能扩增出此片段。结论：此法具有高度的敏感性和特异性。AIM: To study the sensitivity and specificity of nested polymerase chain reaction for detection of Plasmodium vivax in infected mosquitoes. METHODS: Two pairs of primers specific to small subunit ribosomal DNA of P.vivax were used to amplify the specific SSUrDNA 121 bp fragment of P.vivax for detecting P.vivax infected mosquitoes with nested PCR. RESULTS: Nested PCR could detect as few as 3 sporozoites in one mosquito or 1 infected mosquito mixed with a group of 99 normal ones. In contrast, no such specific 121 bp DNA band was detected in P.falciparum, P.cynomolgi, P.yoelii yoelii infected samples, nor in normal mosquito. CONCLUSION: The nested PCR technique we established showed high sensitivity and specificity.

关 键 词：疟原虫 间日疟原虫 蚊虫 套式PCR 

分 类 号：R531.301[医药卫生—内科学] R382.31[医药卫生—临床医学]