氧化修饰脂蛋白对内皮细胞的单核细胞趋化蛋白-1表达的影响  

Effects of oxidatively modified lipoproteins on the expression of monocyte chemoattactant protein 1 in endothelial cells

作　　者：于光耀[1] 邓仲端[1] 瞿智玲[1] 

机构地区：[1]同济医科大学病理学教研室

出　　处：《中华病理学杂志》1998年第3期174-176,共3页Chinese Journal of Pathology

基　　金：国家自然科学基金

摘　　要：目的了解氧化修饰的低密度脂蛋白（ＯＸＬＤＬ）和极低密度脂蛋白（ＯＸＶＬＤＬ）对内皮细胞（ＥＣ）和单核细胞趋化蛋白１（ＭＣＰ１）ｍＲＮＡ和蛋白表达的影响。方法在牛主动脉ＥＣ培养基中分别加入ＬＤＬ、ＯＸＬＤＬ、ＶＬＤＬ和ＯＸＶＬＤＬ，培养２４小时，用异硫氰酸胍法提取细胞的总ＲＮＡ，用γ３２Ｐ末端标记的ＭＣＰ１寡核苷酸探针进行ｄｏｔｂｌｏｔ分析，检测ＥＣ的ＭＣＰ１ｍＲＮＡ的表达。同时用夹心ＥＬＩＳＡ检测各组ＥＣ条件培养基（ＣＭ）中的ＭＣＰ１蛋白含量。用免疫细胞化学法检测ＥＣ的ＭＣＰ１蛋白的表达。结果培养的牛ＥＣ能表达ＭＣＰ１ｍＲＮＡ及蛋白，ＯＸＬＤＬ和ＯＸＶＬＤＬ使其ＭＣＰ１ｍＲＮＡ的表达明显增强，同时使其ＣＭ中ＭＣＰ１蛋白水平增加，而ＬＤＬ和ＶＬＤＬ仅使ＥＣ的ＭＣＰ１ｍＲＮＡ和蛋白的表达轻度增加。免疫细胞化学显示，对照组、ＬＤＬ组和ＶＬＤＬ组ＥＣ胞浆对ＭＣＰ１抗体均呈弱阳性反应，而ＯＸＬＤＬ组和ＯＸＶＬＤＬ组则呈强阳性反应。Objective To clarify whether lipoproteins, particularly oxidatively modified low density lipoprotein (OX LDL) and very low density lipoprotein (OX VLDL) play a role in the express of monocyte chemoattactant protein 1 (MCP 1) mRNA and protein in endothelial cells (ECs). Methods After a 24 hour exposure to LDL, OX LDL, VLDL and OX VLDL respectively the total RNA in calf aorta ECs was extracted by means of guanidinium isothiocyanate method. MCP 1 mRNA expression in ECs was examined by dot blot analysis using a γ 32 P end labelled 35 mer oligonucleotide probe of MCP 1. Meanwhile, MCP 1 protein in EC conditioned media (EC CM) of each group was determined by sandwich ELISA , and MCP 1 protein in ECs was examined immunocytochemically as well. Results Cultured calf aorta ECs expressed MCP 1 mRNA and protein ,and OX LDL and OX VLDL induced further a strong expression of MCP 1 mRNA, and an increased MCP 1 protein level in EC CM. The expression of MCP 1 mRNA and protein was only slightly increased when exposured to LDL and VLDL, and immunohistological staining with polyclonal MCP 1 antibody give a similar result. Conclusion OX LDL and OX VLDL are able to induce a strong expression of MCP 1 in ECs.

关 键 词：脂蛋白 血管内皮 趋化因子 巨噬细胞 冠心病 

分 类 号：R541.402[医药卫生—心血管疾病]