异丙酚对大鼠创伤性脑损伤时DAPKmRNA表达的影响  

Effects of propofol on expression of death-associated protein kinase mRNA in a rat model of traumatic braininjury

作　　者：梁敏[1] 李艳[1] 王宇田[1] 

机构地区：[1]海南省人民医院麻醉科,海口市570311

出　　处：《中华麻醉学杂志》2009年第7期652-654,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：海南省自然科学基金项目（2004-80463）

摘　　要：目的探讨异丙酚对大鼠创伤性脑损伤（TBI）时死亡相关蛋白激酶（DAPK）mRNA表达的影响。方法雄性Wistar大鼠60只，月龄．3—4月，体重250—300g，随机分为6组（n=10）：正常对照组（C组）、假手术组（s组）、TBI组、生理盐水组（NS组）、脂肪乳剂组（FE组）及异丙酚组（P组）。采用自由落体撞击法建立大鼠创伤性脑损伤模型，于制备模型成功后以2ml·kg-1·h-1的速率经尾静脉分别输注生理盐水、10％脂肪乳剂、1％异丙酚4h。于模型制备成功后24h断头处死大鼠取脑，采用细胞原位末端标记（TUNEL）法计数凋亡神经元，计算神经元凋亡率；采用RT-PCR法检测DAPKmRNA的表达水平。结果与c组和s组比较，TBI组模型制备成功后24h时损伤区及损伤边缘区神经元DAPKmRNA表达上调，神经元凋亡率升高（P〈0．05）；P组DAPKmRNA表达水平及神经元凋亡率较TBI组、Ns组和FE组降低（P〈O．05）。结论异丙酚可能通过抑制DAPKmRNA表达上调减少脑神经元凋亡，从而在一定程度上减轻大鼠创伤性脑损伤。Objective To investigate the effects of propofol on death-associated protein kinase （DAPK） mRNA expression in a rat model of traumatic brain injury （TBI）. Methods Sixty male Wistar rats with a mean age of 3-4 months and mean body weight of 250-300 g were randomly divided into 6 groups （ n = 10 each） ： I normal control; Ⅱ sham operation; Ⅲ TBI; Ⅳ normal saline （NS） ＋ TBI; V fat emulsion ＋ TBI and Ⅵ propofol ＋ TBI. TBI was produced according sto Feeney et al. In group m the animals were killed and their brains were removed at 24 h after brain contusion.. NS 2ml·kg-1·h-1, 10% fat emulsion 2 ml·kg-1·h-1 and propofol 2 ml·kg-1·h-1 were infused iv for 4 h after lead trauma in group Ⅳ, Ⅴ and Ⅵ respectively. DAPK mRNA expression in the brain tissue was determined by RT-PCR and neuronal ＇apoptosis ＂was assessed by TUNEL. Results The expression of DAPK mRNA and neuronal apeptosis were significantly increased in the TBI group as compared with normal control and sham operation groups. Propofol infusion significantly attenuated neuronal apoptosis and reduced DAPK mRNA expression as compared with TBI,NS and FE groups. Conclusion Propofol can protect the brain from traumatic injury by suppression of the expression of DAPK mRNA.

关 键 词：二异丙酚 脑损伤 细胞凋亡 神经元 蛋白质丝氨酸苏氨酸激酶 

分 类 号：R96[医药卫生—药理学]