异丙肾上腺素致大鼠心肌肥大时心肌细胞核Ca^(2+)转运功能异常  

Dysfunction of Myocardial Nuclear Calcium Transport in Isoproterenol Induced Rat Myocardial Hypertrophy

作　　者：王培勇[1] 姚兴海[1] 欧和生[1] 苏静怡[1] 汤健[1] 唐朝枢[1] 

机构地区：[1]北京医科大学心血管基础研究所病理生理室,北京100083

出　　处：《中国动脉硬化杂志》1998年第2期98-101,共4页Chinese Journal of Arteriosclerosis

摘　　要：为观察异丙肾上腺素致大鼠心肌肥大时心肌细胞核Ca2+转运功能的改变，给大鼠皮下注射异丙肾上腺素制各心肌肥大模型，采用差速离心和密度梯度离心法分离纯化心肌细胞核，并用酶学方法鉴定核的纯度及测定ATP酶活性，用45Ca2+同位素法测定核钙摄取。结果发现，与对照组相比，实验组肥大心脏的心系数增加40％（P＜0．01），心肌胶原含量增加81％（P＜0．01），Ca2+含量增加41％（P＜0．05），心脏组织呈显著的肥大和纤维化表现。实验组心肌细胞核Ca2+依赖性ATP酶的Ca2+浓度最大反应速度较对照组降低35％（P＜0．01），平衡常数降低46％（P＜0．05），对ATP浓度反应无显著改变，45Ca2+摄取显著低于对照组（P<0.01），其最大反应速度与对照组相比降低62％（P＜0.01），而平衡常数无显著变化。提示异丙肾上腺素所致大鼠的肥大心肌细胞核钙转运功能降低。Aim: Myocardial nuclear caIcium transport was investigated on the model of isoproterenol (ISO) induced cardiac hypertrophy.Methods: Rats were given injections of ISO subcutaneously (20, 10 and 5 mg/kg/day for 3 days, subsequently 3 mg/kg/day for 7 days). Myocardial nuclei were purified with sucrose density centrifugation and identified zymologically, the activity of Ca2+-ATPase was measured zymololgically and calciurn uptake was assayed with 45 Ca2+ isotope.Results: The ratio of heart weight to body weight in ISO group increased by 40% (P<0.01), and ISO-injured heart presented typical cardiac hypertrophy and interstitial fibrosis. Collagen and calcium content in myocardial tissue increased by 81 % and 41 % respectively (P<0.05). Vmax and Km of ATPase activity decreased by about 24% and 46. 1 % respectively (P<0. 05 ). Significant decrease of nuclear Ca2+ uptake was observed and Vmax value was lowered by 62 % compared with that of control(P<0. 01 ), but Km value remained unchanged.Conclusion: The above results indicated a dysfunction of nuclear calcium transport in hypertrophic myocardium.

关 键 词：心肌肥大 CA^2+转运 细胞核 异丙肾上腺素 病理 

分 类 号：R542.02[医药卫生—心血管疾病]