离体鼠心脏短暂缺血后再灌注时脂肪酸代谢的变化  

Metabolic variations of fatty acid in isolated rat heart reperfused after a transient global ischemia

作　　者：黄钢[1] Michel Comet 赵惠扬[2] 朱翠英[1] 袁济民[1] 

机构地区：[1]上海第二医科大学附属仁济医院核医学科 [2]上海医科大学核医学研究所 [3]法国ＪＯＳＥＰＨ,ＦＯＵＲＩＥＲ大学医学院

出　　处：《中华核医学杂志》1998年第3期167-169,共3页Chinese Journal of Nuclear Medicine

基　　金：上海市卫生系统百名学科带头人培养计划资助

摘　　要：目的研究缺血再灌注心脏脂肪酸代谢变化及葡萄糖对其的影响。方法３２只大鼠经麻醉后摘除心脏，并接到Ｌａｎｇｅｎｄｏｒｆ灌注装置上。将离体心脏分成正常组和缺血再灌注组，每组分成２个亚组，即Ｋｒｅｂ灌注液组及Ｋｒｅｂ灌注液加葡萄糖组。在离体心脏的主动脉注入１３１Ｉ十六烷脂肪酸（ＨＡ），并记录心脏的时间放射性曲线。结果正常组离体心脏，灌注液中存在葡萄糖时，脂肪酸代谢清除速率减慢，仅为灌注液中无葡萄糖组的２０％左右。但在缺血再灌注组，即使灌注液中有葡萄糖，再灌注心脏仍表现为脂肪酸分解代谢增加，与灌注液中无葡萄糖组的结果相似，明显高于同灌注条件的正常对照组；然而，灌注液中无葡萄糖时，缺血再灌注心肌的脂肪酸分解代谢低于正常同灌注条件组。结论短暂性缺血对心肌线粒体脂肪酸分解代谢有一定的损害；在正常心脏组中，主要的能量底物为葡萄糖，而缺血后再灌注心肌则以脂肪酸为主要能量底物，可能与再灌注心肌脂肪酸分解代谢代偿性增加，以迅速补充缺血时所造成的能量耗损有关。Purpose The fatty acid metabolism and the effect of glucose on it were studied in isolated and reperfused rat heart Methods 32 isolated working rat hearts were perfused in Langengdorff device with modified krebs and were divided into normal and ischemiareperfused group Each group was also classified into two subgroups, modified krebs with or without glucose subgroup^(131) IHA was injected into aorta of isolated working rat heart and then the radioresidue curves were acquired Results When the isolated rat hearts were perfused with krebs plus glucose, the catabolism of fatty acid was significantly decreased in normal group, but a remarkable increase of fatty acid catabolism was found in ischemiareperfused group While the isolated rat hearts were perfused with krebs without glucose, the catabolism of fatty acid in ischemiareperfused isolated rat heart was less than that in normal group Conclusions Transient ischemia damages the catabolism of myocardial fatty acid in mitochontria in some degree In normal isolated working rat heart, the principal energy source is glucose However, the major energy source is switched to catabolism of fatty acid in ischemiareperfused isolated rat heart This phenomenon may be related to compensative increase of fatty acid catabolism for replenishing the loss of energy during ischemia

关 键 词：心肌缺血 再灌注 脂肪酸代谢 

分 类 号：R542.2[医药卫生—心血管疾病]