地塞米松治疗子宫内膜异位症鸡胚尿囊膜模型的实验研究  

Effects of dexamethasone on endometriosis in chick embryo chorioallantocic membrane model

作　　者：钟洁[1] 何援利[1] 刘木彪[1] 

机构地区：[1]南方医科大学珠江医院妇产科,广州市510282

出　　处：《实用医学杂志》2009年第15期2423-2425,共3页The Journal of Practical Medicine

基　　金：广州市科技计划项目基金资助(编号:2008J-c131)

摘　　要：目的:探讨地塞米松(dexamethasone,DEX)对子宫内膜异位症(endometriosis,EMs)血管生成的抑制作用,为抗血管途径治疗EMs提供依据。方法:将EMs患者在位子宫内膜碎屑接种于8d胚龄的鸡胚绒毛尿囊膜(CAM)上,建立子宫内膜异位症鸡胚尿囊膜模型(EMsCAM)。分别设定接种组、接种治疗组(DEX)和空白组。利用Image-ProPlus6.0软件,定量血管新生面积、蛋壳开窗处对应的CAM面积,计算出血管新生面积与CAM面积的比值。结果:接种组CAM血管生成反应明显增强,血管面积比(VA/CAM)明显大于空白组;接种治疗组VA/CAM明显小于接种组。结论:EMs在位子宫内膜能明显促进CAM的血管生成,为EMs异位种植和存活理论的研究提供一个理论依据。地塞米松能显著抑制异位病灶的血管形成,是抗血管途径治疗EMs的有效策略之一。Objective To investigate the depressant effect of dexamethasone on angiogenesis of endometriosis. Methods This research included 3 groups （vaccination group, vaccination treating group, and control group）. Eutopic endometrium of women with endometriosis was transplanted to the non-vascular region of the 8-day-old chick embryo chorioallantocic membrane （CAM） in vaccination group and vaccination treating group. Image-Pro Plus 6.0 software was used to quantify the angiogenesis area （VA）, the total CAM area under the egg shell opening, and the ratio of them （VA/CAM）. Results A strong angiogenic response was observed in vaccination group, and the VA/CAM ratio in this group was significantly higher than those in control group and vaccination treating group. Conclusion Eutopic endometrium of endometriosis can induce angiogenesis, but dexamethasone can inhibit this effect.

关 键 词：子宫内膜异位症 鸡胚绒毛尿囊膜 地塞米松 

分 类 号：R96[医药卫生—药理学]