低频电磁场对大鼠生精细胞凋亡基因表达影响  

Effect of extremely low frequency electromagnetic fields on Bax and Bcl-2 expression in spermatogenic cells of rats

作　　者：孙晓芳[1] 段斐[1] 寇素茹[1] 刘晋芝[1] 马幼敏[1] 

机构地区：[1]河北大学基础医学院组胚教研室,河北保定071000

出　　处：《中国公共卫生》2009年第8期989-990,共2页Chinese Journal of Public Health

基　　金：河北省计划生育委员会科技攻关计划项目(06-04)

摘　　要：目的探讨极低频电磁场对大鼠生精细胞凋亡相关基因Bax和Bcl-2表达的影响。方法Wistar大鼠60只随机分为低剂量(0.1mT)、高剂量(12.8mT)电磁场暴露组及对照组,每组20只,分别有10只持续暴露2和12周;用苏木苏-伊红(H-E)染色及免疫组织化学法观察睾丸形态学和细胞凋亡相关基因Bax、Bcl-2表达的变化,用图像分析系统进行测定。结果低、高剂量电磁场暴露组暴露2周Bax灰度值分别为(25.58±4.98),(39.17±4.33);Bcl-2灰度值分别为(68.12±3.64),(39.62±4.35);暴露12周Bax灰度值分别为(40.12±2.45),(62.32±5.90);Bcl-2灰度值分别为(55.78±2.43)(23.84±3.62)。结论随着电磁场暴露频率的升高和时间的延长,大鼠生精细胞Bax的表达明显上调,Bcl-2的表达逐渐下调。Objective To study the effect of extremely low frequency electromagnetic fields on Bax and Bcl-2 expression in the spermatogenic cells of rats. Methods Sixty adult female Wistar rats were divided into 0.1 mT, 12.8 mT and control group. After electromagnetic radiation for 2 and 12 weeks,HE staining and immunohistochemical methods were used to detected Bar and Bcl-2 expression with image analysis. Results After electromagnetic exposure for 2 week, the gray scale values of Bax were 25. 58 ±4. 98 and 39. 17 ±4. 33 for low and high intensity groups and that of Bcl-2 were 68. 12± 3.64 and 39. 62 ± 4.35. The gray scale values of Box were 40.12 ± 2.45 and 62.32 ± 5.90 for low and high intensity groups for 12 weeks exposure and that of Bcl-2 were 55.7 ±2.43 and 23.84 ± 3.62. Conclusion With the increase of exposure intensity and time, the Bax and Bcl-2 exprseeion in spermatogenic cells in rats were gradually decreased.

关 键 词：极低频电磁场 生精细胞 凋亡相关基因 Bax Bcl-2 

分 类 号：R122.4[医药卫生—环境卫生学]