豆类丝核菌次级代谢产物对小鼠的一般生殖毒性  

General Reproductive Toxicity Study of Secondary Metabolites of Rhizotonia leguminicola on Mouse

作　　者：张伟[1] 杨鸣琦[1] 郭卫华[1] 于路芹 李锡杰[1] 戴卉敏[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]天津市宝坻区畜牧水产局,天津宝坻301800

出　　处：《西北农业学报》2009年第4期70-73,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(30270979)

摘　　要：研究豆类丝核菌次级代谢产物对小鼠的一般生殖毒性。昆明种小鼠80只,随机分为4组,每组20只,雌雄各半。设一个空白对照组和3个试验组,给药剂量分别为56.62、14.15和3.54 mg/kg。交配前雄鼠连续给药28 d,雌鼠连续给药14 d,雌雄按1∶1合笼交配,发现阴道栓之日定为受孕始日,交配后雌鼠继续给药13 d。结果表明,56.62 mg/kg对雄鼠、雌鼠、仔鼠均有影响,表现为雄鼠附睾质量(P<0.01)、附睾系数(P<0.05)、精子总数(P<0.01)的异常;雌鼠体质量的下降(P<0.05)以及仔鼠第4天成活率的降低(P<0.05)。14.15 mg/kg使雄鼠附睾质量(P<0.01)和附睾系数(P<0.05)降低并造成精子总数增多(P<0.05)。3.54 mg/kg对所有指标无显著影响(P>0.05)。因此,在本试验条件下,小鼠一般生殖毒性无作用剂量为3.54 mg/kg,相当于肿瘤有效治疗量的1.31倍。To study the general reproductive toxicity of secondary metabolites of Rhizotonia leguminicola on mouse. 80 KM mices were randomly divided into 4 groups, each group 20, female and male own a half respectively. Design 1 control group and 3 test groups, 56.62 mg/kg, 14.15 mg/kg, 3.54 mg/kg is used in every test groups. Affuse to male mouse 28 days before mating, female mouse 14 days, 1 ： 1 mating, the day finding vagina cork as the 0 d of pregnancy. After mating, affuse 13days to the female continually. The result showed that there are impacts of 56.62mg/kg to male mouse,female mouse,young mouse, by the abnormity of weight of epididymis（P〈0.01） and epididymis coefficient （P〈0.05） as well as the total number of sperm（P〈0.01）. The weight of female mouse（P〈0.05） and the 4 day survival rate of young mouse（P〈0.05） are low. 14.15 mg/kg leads to the reduce of weight of epididymis（P〈0.01） and epididymis coefficient （P〈0.05） , results into the growth of total number of sperm（P〈0.05）. There is not remarkable impact on all index of 3.54 mg/kg （P〉0. 05）. Consequently, under the situation of this test, the observed adverse effect level （NOAEL） of general reproductive toxicity is about 3.54mg/kg in mouse, as 1.31 times of the effective treatment dose of tumor.

关 键 词：豆类丝核菌 次级代谢产物 苦马豆素 一般生殖毒性 

分 类 号：S567.39[农业科学—中草药栽培]