RP-HPLC法测定战创伤涂膜剂中3组分的含量  

Determination of Three Kinds of Components in Film Forming Solution for War Wound and Trauma by HPLC

作　　者：戴卫红[1] 李云霞[1] 刘建芳[1] 张素芬[1] 侯艳宁[1] 

机构地区：[1]解放军白求恩国际和平医院,石家庄050082

出　　处：《中国药师》2009年第8期1001-1003,共3页China Pharmacist

基　　金：全军十一五科技攻关课题(06G024)

摘　　要：目的:建立高效液相色谱法测定战创伤涂膜剂中替硝唑、盐酸达克罗宁和醋酸氯己定3组分的含量。方法:色谱柱为Diamonsil C_(18)(150 mm×4.6 mm,5μm);流动相:甲醇-0.02 mol·L^(-1)磷酸二氢钾水溶液(含三乙胺0.4%,用磷酸调节pH至2.5)(50:50);流速:1.0 ml·min^(-1);检测波长分别为317 nm,280 nm,261 nm;柱温:25℃。结果:替硝唑、盐酸达克罗宁和醋酸氯己定的线性范围分别为1.02～10.20,12.48～124.80和5.42～54.20μg·ml^(-1),相关系数均为1.0000;平均回收率分别为101.6%、100.1%和102.3%。结论:本法简便准确,重复性好,可用于战创伤涂膜剂的质量控制。Objective ： To establish an HPLC method for the determination of tinidazol, dyclonine hydrochloridum and chlorhexidine acetate in film forming solution for war wound and trauma. Method：The HPLC separation was conducted on a Diamonsil C18 co umn（ 150 mm ×4.6 mm,5μm） , the mobile phase consisted of methanol and 0.02 mol · L^-1 potassium dihydrogen phosphate solution （ contained 0.4 % triethylamine, and adjust pH to 2.5 with phosphoric acid ） （ 50 ： 50 ）, the flow rate was 1.0 ml · min ^-1, the detection wavelengths were 317,280, and 261 nm, respectively, and the column temperature was 25 ℃. Result： The linear ranges of tinidazol, dyclonine hydrochloridum and chlorhexidine acetate were 1.02 -10.20 μg · ml^-1 （ r = 1. 000 0 ） , 12.48-124.83 μg · ml^-1 （ r = 1. 000 0 ） and 5.42 - 54.20 μg · ml^ - 1 （ r = 1. 000 0 ） respectively, the average recoveries were 101.6% , 100.1%, and 102.3 %, respectively. Conclusion： The method is accurate and reproducible. It can be used for the quality control of the studied film forming solution for war wound and trauma.

关 键 词：高效液相色谱法 涂膜剂 替硝唑 盐酸达克罗宁 醋酸氯己定 

分 类 号：R927.2[医药卫生—药学]