缺氧时肺动脉内皮细胞与肺动脉平滑肌细胞相互作用对细胞内环核苷酸的影响  

Modulations of cyclic nucleotides pulmonary artery endothelial cells and smooth muscle cells under hypoxia in vitro

作　　者：王培勇[1] 刘健[1] 于中和[1] 许蜀闽[1] 王俊元[1] 孙秉庸[1] 

机构地区：[1]第三军医大学高原医学研究室病理生理教研室 [2]北京医科大学心血管病基础研究所 [3]北京军区总医院呼吸内科 [4]重庆新桥医院心内科

出　　处：《中国病理生理杂志》1998年第4期351-354,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：目的：观察了培养的小牛肺动脉内皮细胞（ＰＡＥＣ）和肺动脉平滑肌细胞（ＰＡＳＭ）于缺氧时对细胞内环核苷酸的影响。结果：ＰＡＥＣ和ＰＡＳＭ共培养２４ｈ，ＰＡＥＣ细胞内ｃＡＭＰ含量显著降低（Ｐ＜００１），而ＰＡＳＭ细胞内ｃＡＭＰ含量显著增加（Ｐ＜００１），二种细胞内ｃＧＭＰ含量均显著降低（Ｐ＜００１）。缺氧对二种细胞内ｃＡＭＰ含量无显著影响，但能增加ＰＡＳＭ的ｃＧＭＰ含量（Ｐ＜００１），降低ＰＡＥＣ的ｃＧＭＰ含量（Ｐ＜００１）。ＮＯ合酶抑制剂硝基精氨酸对二种细胞的ｃＡＭＰ含量均无显著影响，但能使常氧培养的ＰＡＥＣ和缺氧培养的ＰＡＳＭ细胞内的ｃＧＭＰ含量显著降低（Ｐ＜００１）。结论：ＰＡＥＣ和ＰＡＳＭ的相互作用可引起第二信使系统传递的变化；AIM :Interactions between cultured new bovine pulmonary arterial endothelial cell (PAEC) and pulmonary arterial smooth muscle cell (PASM) under hypoxic conditions were investigated.Content of cyclic nucleotides were assayed by RIA.RESULTS:When PAEC and PASM were cocultured in normoxic and hypoxic conditions for 24h,cAMP content decreased in PAEC and increased in PASM(P<001),cGMP content decreased in both types of cells(P<001).NO synthase inhibitor L-nitro-arginine exerted inhibitory effect on cGMP production in normoxic cultured PAEC and hypoxic cultured PASM(P<001).CONCLUSION:PAECs and PASMs may regulate each other by second massenger system,and hypoxia may induce NO synthase in PASM but inhibit NO synthase in PAEC.It was proposed that such changes may participate in the development of hypoxic pulmonary hypertension. 

关 键 词：缺氧 肺动脉 环核苷酸 内皮细胞 平滑肌细胞 

分 类 号：R594.302[医药卫生—内科学] R339.54[医药卫生—临床医学]